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We have studied the binding of proteins to a synthetic DNA fragment corresponding to a 21 base pair sequence in the upstream region of the human c-myc gene. This sequence has previously been reported to bind the c-myc protein, thus representing a putative site for autoregulation of the c-myc gene (Ariga et al., 1989). Our electrophoretic mobility shift assays indicated that this DNA fragment binds to a factor present in nuclear extracts from HeLa cells. However, analysis of extracts from other cells indicated that this binding does not correlate with the presence of the c-myc protein. No binding was observed using c-myc protein translated in vitro. Also, anti-c-myc antibodies could not inhibit binding of this factor. These experiments indicate, that nucleotides -2193 to -2173 from the P1 promoter of the c-myc gene are able to bind a nuclear factor, and that this factor most likely does not include the c-myc protein.
K Saksela, and
P J Koskinen, and
K Alitalo
November 2000,
Nucleic acids research,
K Saksela, and
P J Koskinen, and
K Alitalo
October 1988,
Molecular and cellular biology,
K Saksela, and
P J Koskinen, and
K Alitalo
December 1988,
Oncogene,
K Saksela, and
P J Koskinen, and
K Alitalo
April 2009,
Blood,
K Saksela, and
P J Koskinen, and
K Alitalo
January 1987,
Progress in clinical and biological research,
K Saksela, and
P J Koskinen, and
K Alitalo
November 1984,
Proceedings of the National Academy of Sciences of the United States of America,
K Saksela, and
P J Koskinen, and
K Alitalo
February 1993,
Biochemical and biophysical research communications,
K Saksela, and
P J Koskinen, and
K Alitalo
April 1994,
Oncogene,
K Saksela, and
P J Koskinen, and
K Alitalo
November 1994,
International journal of oncology,
K Saksela, and
P J Koskinen, and
K Alitalo
February 2000,
Biochemistry,
