-transparent.png){kind=logo}
N-Linked sugar chains were liberated by hydrazinolysis from porcine zona pellucida glycoproteins obtained from ovarian follicular oocytes. Neutral sugar chains were separated from acidic ones by paper electrophoresis and fractionated with a serial lectin column chromatography and Bio-Gel P-4 column chromatography. Their structural analysis by sequential glycosidase digestion in combination with methylation analysis revealed that the neutral sugar chains are of bi-, tri-, and tetraantennary complex type with a fucosylated trimannosyl core. Twenty-six percent of the sugar chains contain N-acetyllactosamine repeating structures in their outer chain moieties. Only linear N-acetyllactosamine repeats, the maximum size of which is hexasaccharide, are detected. A characteristic feature is that 39% of the sugar chains contain N-acetylglucosamine residues at their nonreducing termini in spite of the absence of bisected sugar chains. This study provided, for the first time, the substantial information about the sugar chain structures of mammalian zona pellucida glycoproteins.
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
June 1993,
European journal of biochemistry,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
April 1994,
European journal of biochemistry,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
August 1993,
FEBS letters,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
January 1988,
Annual review of biochemistry,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
September 2008,
The Journal of biological chemistry,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
October 1989,
Biochemistry,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
April 1985,
Biology of reproduction,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
February 2005,
Molecular reproduction and development,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
June 1999,
Molecular human reproduction,
E Mori, and
S Takasaki, and
J L Hedrick, and
N J Wardrip, and
T Mori, and
A Kobata
March 1997,
Molecular reproduction and development,
