BIOMAT Database Logo BIOMAT Database Logo

Pitfalls in measuring high-density lipoprotein cholesterol concentrations in HIV-infected patients. 2010

G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Centre de Recerca Biomèdica, Hospital Universitari Sant Joan, Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Reus, Spain.

OBJECTIVE HIV infection and its treatment are associated with dyslipidaemia and increased risk of cardiovascular disease. Accurate high-density lipoprotein (HDL) cholesterol values are necessary for the management of these abnormalities, but current methods have not been properly assessed in these patients. The aim of this study was to assess in HIV-infected patients the consistency and accuracy of a synthetic polymer/detergent homogeneous assay used to measure HDL cholesterol concentrations and to evaluate the impact of storage. METHODS HDL cholesterol was measured using a synthetic polymer/detergent homogeneous method in samples from HIV-infected patients and healthy subjects for each of the storage regimens: baseline, after 1 week at 4 degrees C, and after 12 months at -80 degrees C. The ultracentrifugation and precipitation assays were used for comparison. RESULTS Three out of every 20 samples from HIV-infected patients had discrepant HDL cholesterol values with respect to the ultracentrifugation method. Overestimation was associated with high C-reactive protein concentrations and underestimation with plasma gamma-globulin concentrations, an effect that was amplified by any of the storage conditions tested. CONCLUSIONS Caution is needed when using the synthetic polymer/detergent homogeneous method for direct measurement of HDL cholesterol concentrations in HIV-infected patients. This assay is of limited use in clinical trials in which frozen samples are analysed.

UI MeSH Term Description Entries
D008076 Cholesterol, HDL Cholesterol which is contained in or bound to high-density lipoproteins (HDL), including CHOLESTEROL ESTERS and free cholesterol. High Density Lipoprotein Cholesterol,Cholesterol, HDL2,Cholesterol, HDL3,HDL Cholesterol,HDL(2) Cholesterol,HDL(3) Cholesterol,HDL2 Cholesterol,HDL3 Cholesterol,alpha-Lipoprotein Cholesterol,Cholesterol, alpha-Lipoprotein,alpha Lipoprotein Cholesterol
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D011108 Polymers Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS). Polymer
D011232 Chemical Precipitation The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution. Precipitation, Chemical
D011933 Reagent Kits, Diagnostic Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use. Diagnostic Reagent Kits,Diagnostic Reagents and Test Kits,Diagnostic Test Kits,In Vitro Diagnostic Device,In Vitro Diagnostic Devices,In Vitro Diagnostic Medical Device,In Vitro Diagnostic Medical Devices,Kits, Diagnostic Reagent,Diagnostic Reagent Kit,Diagnostic Test Kit,Kit, Diagnostic Reagent,Kit, Diagnostic Test,Kits, Diagnostic Test,Reagent Kit, Diagnostic,Test Kit, Diagnostic,Test Kits, Diagnostic
D002097 C-Reactive Protein A plasma protein that circulates in increased amounts during inflammation and after tissue damage. C-Reactive Protein measured by more sensitive methods often for coronary heart disease risk assessment is referred to as High Sensitivity C-Reactive Protein (hs-CRP). High Sensitivity C-Reactive Protein,hs-CRP,hsCRP,C Reactive Protein,High Sensitivity C Reactive Protein
D003627 Data Interpretation, Statistical Application of statistical procedures to analyze specific observed or assumed facts from a particular study. Data Analysis, Statistical,Data Interpretations, Statistical,Interpretation, Statistical Data,Statistical Data Analysis,Statistical Data Interpretation,Analyses, Statistical Data,Analysis, Statistical Data,Data Analyses, Statistical,Interpretations, Statistical Data,Statistical Data Analyses,Statistical Data Interpretations
D005188 False Negative Reactions Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed) False Negative Reaction,Reaction, False Negative,Reactions, False Negative
D005260 Female Females

Related Publications

G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Factors affecting high-density lipoprotein cholesterol in HIV-infected patients on nevirapine-based antiretroviral therapy.
May 2017, The Indian journal of medical research,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Simplified methods for measuring cholesterol concentrations of high-density lipoprotein subclasses in serum compared.
July 1986, Clinical chemistry,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Relationship between high-density lipoprotein-cholesterol and malondialdehyde-modified low-density lipoprotein concentrations.
January 2003, Journal of atherosclerosis and thrombosis,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Serum high-density lipoprotein cholesterol concentrations in hypo- and hyperthyroidism.
April 1980, Clinical chemistry,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Relationship between total cholesterol/high-density lipoprotein cholesterol ratio, triglyceride/high-density lipoprotein cholesterol ratio, and high-density lipoprotein subclasses.
September 2006, Metabolism: clinical and experimental,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Clinical significance of high-density lipoprotein cholesterol in patients with low low-density lipoprotein cholesterol.
January 2008, Journal of the American College of Cardiology,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
High-density lipoprotein cholesterol.
January 1979, The Medical journal of Australia,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Heated tobacco products and circulating high-density lipoprotein cholesterol concentrations.
October 2022, Scientific reports,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
Association of the low-density lipoprotein cholesterol/high-density lipoprotein cholesterol ratio and concentrations of plasma lipids with high-density lipoprotein subclass distribution in the Chinese population.
July 2010, Lipids in health and disease,
G Aragonès, and R Beltrán, and A Rull, and J Marsillach, and F Rodríguez, and S B Alí, and L Fernández-Sender, and J Camps, and J Joven, and C Alonso-Villaverde
High density lipoprotein cholesterol concentrations in physically active and sedentary spinal cord injured patients.
July 1986, Archives of physical medicine and rehabilitation,
Copied contents to your clipboard!