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Delivery of cloned offspring: experience in Zebu cattle (Bos indicus). 2010
Flávio V Meirelles, and
Eduardo H Birgel, and
Felipe Perecin, and
Marcelo Bertolini, and
Anneliese S Traldi, and
José Rodrigo V Pimentel, and
Eliza R Komninou, and
Juliano R Sangalli, and
Paulo Fantinato Neto, and
Mariana Tikuma Nunes, and
Fábio Celidonio Pogliani, and
Flávia D P Meirelles, and
Flávia S Kubrusly, and
Camila I Vannucchi, and
Liege C G Silva
Faculty of Animal Sciences and Food Engineering, University of São Paulo, Av. Duque de Caxias Norte, 225. Pirassununga, SP 13635-900, Brazil. meirellf@usp.br
The production of a healthy cloned calf is dependent on a multitude of successful steps, including reprogramming mediated by the oocyte, the development of a functional placenta, adequate maternal-fetal interaction, the establishment of a physiological metabolic setting and the formation of a complete set of well-differentiated cells that will eventually result in well-characterised and fully competent tissues and organs. Although the efficiency of nuclear transfer has improved significantly since the first report of a somatic cell nuclear transfer-derived animal, there are many descriptions of anomalies concerning cloned calves leading to high perinatal morbidity and mortality. The present article discusses some our experience regarding perinatal and neonatal procedures for cloned Zebu cattle (B. indicus) that has led to improved survival rates in Nellore cloned calves following the application of such 'labour-intensive technology'.
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