Parasympathectomy induces morphological changes and alters gene-expression profiles in the rat submandibular gland. 2010

J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
2nd Stage BK 21 for School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju 500-757, South Korea.

OBJECTIVE The chorda-lingual (CL) nerve carries parasympathetic fibers to the hilum of the sublingual and submandibular glands (SMGs) and evokes the secretion of saliva. The effect of cutting the CL nerve on the biological processes in SMGs was investigated by examining the gene-expression profiles in the SMGs after a surgical parasympathectomy. METHODS At day 3 after the CL nerve cut, the changes in the SMGs at both the experimental cut and contralateral control sides were analysed by microarray and light microscopy. The expression levels of 6 selected genes were confirmed by real-time PCR, Western blot and immunofluorescence staining. RESULTS The wet weight of the parasympathectomised SMGs decreased significantly compared to that of the contralateral side (p<0.05). Histological analyses after the parasympathectomy showed a widened interacinar space as well as some atropic changes to the acini of the SMGs in the cut side. Microarray analysis revealed that twofold differential expression in mRNA expression in the parasympathectomized SMGs were detected in 88 genes (0.004%): 41 genes were overexpressed, 11 were underexpressed and 36 were unknown. Changes of the expression of 6 selected genes detected by Western blot and/or real-time PCR were consistent with the microarray data. CONCLUSIONS The important genes involved in biological processes for salivation were identified through a large-scale gene expression analysis.

UI MeSH Term Description Entries
D007124 Immunoenzyme Techniques Immunologic techniques based on the use of: (1) enzyme-antibody conjugates; (2) enzyme-antigen conjugates; (3) antienzyme antibody followed by its homologous enzyme; or (4) enzyme-antienzyme complexes. These are used histologically for visualizing or labeling tissue specimens. Antibody Enzyme Technique, Unlabeled,Enzyme Immunoassay,Enzyme-Labeled Antibody Technique,Immunoassay, Enzyme,Immunoperoxidase Techniques,Peroxidase-Antiperoxidase Complex Technique,Peroxidase-Labeled Antibody Technique,Antibody Enzyme Technic, Unlabeled,Enzyme-Labeled Antibody Technic,Immunoenzyme Technics,Immunoperoxidase Technics,Peroxidase-Antiperoxidase Complex Technic,Peroxidase-Labeled Antibody Technic,Antibody Technic, Enzyme-Labeled,Antibody Technic, Peroxidase-Labeled,Antibody Technics, Enzyme-Labeled,Antibody Technics, Peroxidase-Labeled,Antibody Technique, Enzyme-Labeled,Antibody Technique, Peroxidase-Labeled,Antibody Techniques, Enzyme-Labeled,Antibody Techniques, Peroxidase-Labeled,Enzyme Immunoassays,Enzyme Labeled Antibody Technic,Enzyme Labeled Antibody Technique,Enzyme-Labeled Antibody Technics,Enzyme-Labeled Antibody Techniques,Immunoassays, Enzyme,Immunoenzyme Technic,Immunoenzyme Technique,Immunoperoxidase Technic,Immunoperoxidase Technique,Peroxidase Antiperoxidase Complex Technic,Peroxidase Antiperoxidase Complex Technique,Peroxidase Labeled Antibody Technic,Peroxidase Labeled Antibody Technique,Peroxidase-Antiperoxidase Complex Technics,Peroxidase-Antiperoxidase Complex Techniques,Peroxidase-Labeled Antibody Technics,Peroxidase-Labeled Antibody Techniques,Technic, Enzyme-Labeled Antibody,Technic, Immunoenzyme,Technic, Immunoperoxidase,Technic, Peroxidase-Antiperoxidase Complex,Technic, Peroxidase-Labeled Antibody,Technics, Enzyme-Labeled Antibody,Technics, Immunoenzyme,Technics, Immunoperoxidase,Technics, Peroxidase-Antiperoxidase Complex,Technics, Peroxidase-Labeled Antibody,Technique, Enzyme-Labeled Antibody,Technique, Immunoenzyme,Technique, Immunoperoxidase,Technique, Peroxidase-Antiperoxidase Complex,Technique, Peroxidase-Labeled Antibody,Techniques, Enzyme-Labeled Antibody,Techniques, Immunoenzyme,Techniques, Immunoperoxidase,Techniques, Peroxidase-Antiperoxidase Complex,Techniques, Peroxidase-Labeled Antibody
D008297 Male Males
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D012472 Salivation The discharge of saliva from the SALIVARY GLANDS that keeps the mouth tissues moist and aids in digestion.
D013363 Submandibular Gland One of two salivary glands in the neck, located in the space bound by the two bellies of the digastric muscle and the angle of the mandible. It discharges through the submandibular duct. The secretory units are predominantly serous although a few mucous alveoli, some with serous demilunes, occur. (Stedman, 25th ed) Submaxillary Gland,Gland, Submandibular,Gland, Submaxillary,Glands, Submandibular,Glands, Submaxillary,Submandibular Glands,Submaxillary Glands
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D017207 Rats, Sprague-Dawley A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company. Holtzman Rat,Rats, Holtzman,Sprague-Dawley Rat,Rats, Sprague Dawley,Holtzman Rats,Rat, Holtzman,Rat, Sprague-Dawley,Sprague Dawley Rat,Sprague Dawley Rats,Sprague-Dawley Rats
D017764 Parasympathectomy The removal or interruption of some part of the parasympathetic nervous system for therapeutic or research purposes. Denervation, Parasympathetic,Parasympathetic Denervation,Denervations, Parasympathetic,Parasympathectomies,Parasympathetic Denervations
D046228 Microarray Analysis The simultaneous analysis, on a microchip, of multiple samples or targets arranged in an array format. Microarray Analytical Devices,Microarray Microchips,Nanoarray Analytical Devices,Analysis, Microarray,Analytical Device, Microarray,Analytical Device, Nanoarray,Analytical Devices, Microarray,Analytical Devices, Nanoarray,Device, Microarray Analytical,Device, Nanoarray Analytical,Devices, Microarray Analytical,Devices, Nanoarray Analytical,Microarray Analytical Device,Microarray Microchip,Microchip, Microarray,Microchips, Microarray,Nanoarray Analytical Device

Related Publications

J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
January 1990, Archives of oral biology,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
March 2005, Journal of neuroimmunology,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
June 1983, Hiroshima Daigaku shigaku zasshi. The Journal of Hiroshima University Dental Society,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
January 1986, Revista da Faculdade de Odontologia de Ribeirao Preto,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
January 1989, Gerodontology,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
September 2011, Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
October 2002, European journal of morphology,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
January 1996, European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
June 2017, Brain research bulletin,
J H Kang, and B K Kim, and B I Park, and H J Kim, and H M Ko, and S Y Yang, and M S Kim, and J Y Jung, and W J Kim, and W M Oh, and S H Kim, and J H Kim
February 2003, European journal of morphology,
Copied contents to your clipboard!