BIOMAT Database Logo BIOMAT Database Logo

Technique for collection of cerebrospinal fluid from the cisterna magna in rat. 2010

Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
Department of Biological Science, ISU, Normal, IL, USA.

Cerebrospinal fluid (CSF) is in direct contact with the extracellular space in the central nervous system (CNS), and biological changes in the brain can be reflected in CSF. In the present article, a procedure for collection of CSF in rats is described. The technique quickly and reliably yields large quantities of CSF (50-150 microl) in rats. More importantly, blood contamination of the CSF is avoided. Furthermore, detections of ATP and interleukin (IL)-1 beta in the CSF have been carried out. ATP concentration in the CSF samples was between 8.3 and 15.8 nM, with an average of 10.5+/-0.83 nM (mean+/-SEM). The concentrations of IL-1beta were below the detection limit in the CSF in the laminectomy control rats, but it increased to 0.26+/-0.07 ng/ml at 1h after spinal cord injury. This technique offers an alternative method to surgical cannulation for the collection of CSF in rats.

UI MeSH Term Description Entries
D007796 Laminectomy A surgical procedure that entails removing all (laminectomy) or part (laminotomy) of selected vertebral lamina to relieve pressure on the SPINAL CORD and/or SPINAL NERVE ROOTS. Vertebral lamina is the thin flattened posterior wall of vertebral arch that forms the vertebral foramen through which pass the spinal cord and nerve roots. Laminotomy,Laminectomies,Laminotomies
D008297 Male Males
D009483 Neuropsychological Tests Tests designed to assess neurological function associated with certain behaviors. They are used in diagnosing brain dysfunction or damage and central nervous system disorders or injury. Aphasia Tests,Cognitive Test,Cognitive Testing,Cognitive Tests,Memory for Designs Test,Neuropsychological Testing,AX-CPT,Behavioral Assessment of Dysexecutive Syndrome,CANTAB,Cambridge Neuropsychological Test Automated Battery,Clock Test,Cognitive Function Scanner,Continuous Performance Task,Controlled Oral Word Association Test,Delis-Kaplan Executive Function System,Developmental Neuropsychological Assessment,Hooper Visual Organization Test,NEPSY,Neuropsychologic Tests,Neuropsychological Test,Paced Auditory Serial Addition Test,Repeatable Battery for the Assessment of Neuropsychological Status,Rey-Osterrieth Complex Figure,Symbol Digit Modalities Test,Test of Everyday Attention,Test, Neuropsychological,Tests, Neuropsychological,Tower of London Test,Neuropsychologic Test,Test, Cognitive,Testing, Cognitive,Testing, Neuropsychological,Tests, Cognitive
D001769 Blood The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D001933 Brain Stem The part of the brain that connects the CEREBRAL HEMISPHERES with the SPINAL CORD. It consists of the MESENCEPHALON; PONS; and MEDULLA OBLONGATA. Brainstem,Truncus Cerebri,Brain Stems,Brainstems,Cerebri, Truncus,Cerebrus, Truncus,Truncus Cerebrus
D002555 Cerebrospinal Fluid A watery fluid that is continuously produced in the CHOROID PLEXUS and circulates around the surface of the BRAIN; SPINAL CORD; and in the CEREBRAL VENTRICLES. Cerebro Spinal Fluid,Cerebro Spinal Fluids,Cerebrospinal Fluids,Fluid, Cerebro Spinal,Fluid, Cerebrospinal,Fluids, Cerebro Spinal,Fluids, Cerebrospinal,Spinal Fluid, Cerebro,Spinal Fluids, Cerebro
D002946 Cisterna Magna One of three principal openings in the SUBARACHNOID SPACE. They are also known as cerebellomedullary cistern, and collectively as cisterns. Magna, Cisterna
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D000255 Adenosine Triphosphate An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter. ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
A technique for serial collection of cerebrospinal fluid from the cisterna magna in mouse.
November 2008, Journal of visualized experiments : JoVE,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
An improved technique for cerebrospinal fluid collection of cisterna magna in Rhesus monkeys.
July 2015, Journal of neuroscience methods,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
Technique for repeated collection of cerebrospinal fluid from cisterna magna of anesthetized strain 13 guinea pigs.
September 1991, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
A step-by-step guide for microsurgical collection of uncontaminated cerebrospinal fluid from rat cisterna magna.
March 2021, Journal of neuroscience methods,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
New method for the collection of cerebrospinal fluid from the cisterna magna of conscious dogs.
April 1984, The American journal of physiology,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
Technique for collecting cerebrospinal fluid in the cisterna magna of non-anesthetized rats.
February 2000, Brain research. Brain research protocols,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
A technique for cannulating the Cisterna magna and sampling cerebrospinal fluid from socially housed birds.
April 1994, Poultry science,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
An alternative method of chronic cerebrospinal fluid collection via the cisterna magna in conscious rhesus monkeys.
July 2003, Contemporary topics in laboratory animal science,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
[Physiological variations in cerebrospinal fluid pressure in the cisterna magna].
March 1963, Arquivos de neuro-psiquiatria,
Christina C Pegg, and Chunyan He, and Ann R Stroink, and Keith A Kattner, and Chen Xu Wang
Chronic Collection of Cerebrospinal Fluid from Rhesus Macaques (Macaca mulatta) with Cisterna Magna Ports: Update on Refinements.
February 2022, Comparative medicine,
Copied contents to your clipboard!