Antimicrobial and immunomodulatory activities of an ovine proline/arginine-rich cathelicidin. 2010

Pak-Lam Yu, and Martin L Cross, and Richard G Haverkamp
Biotechnology Group, School of Engineering and Advanced Technology, Massey University, Private Bag 11-222, Palmerston North 4442, New Zealand. P.Yu@massey.ac.nz

In in vitro co-culture experiments, the ovine-derived cathelicidin OaBac5mini showed antimicrobial activity against Escherichia coli cells and modulated production of a cytokine by a mammalian inflammatory cell type (macrophage). Using atomic force microscopy, the morphology of peptide-treated E. coli bacteria showed no cell lysis, indicating an intracellular mode of action of the peptide leading to bacterial cell inhibition. At a concentration of 50microg/mL OaBac5mini, the peptide suppressed production of the inflammatory cytokine interleukin-12 by murine J774A cells that had been stimulated with Staphylococcus aureus strain Cowan; levels of other cytokines were unaffected. Thus, certain cationic peptides can enter and disrupt invading Gram-negative pathogens and may be able to modulate inflammatory responses induced by Gram-positive bacterial products.

UI MeSH Term Description Entries
D007155 Immunologic Factors Biologically active substances whose activities affect or play a role in the functioning of the immune system. Biological Response Modifier,Biomodulator,Immune Factor,Immunological Factor,Immunomodulator,Immunomodulators,Biological Response Modifiers,Biomodulators,Factors, Immunologic,Immune Factors,Immunological Factors,Modifiers, Biological Response,Response Modifiers, Biological,Factor, Immune,Factor, Immunological,Factors, Immune,Factors, Immunological,Modifier, Biological Response,Response Modifier, Biological
D008264 Macrophages The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.) Bone Marrow-Derived Macrophages,Monocyte-Derived Macrophages,Macrophage,Macrophages, Monocyte-Derived,Bone Marrow Derived Macrophages,Bone Marrow-Derived Macrophage,Macrophage, Bone Marrow-Derived,Macrophage, Monocyte-Derived,Macrophages, Bone Marrow-Derived,Macrophages, Monocyte Derived,Monocyte Derived Macrophages,Monocyte-Derived Macrophage
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D012756 Sheep Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS. Ovis,Sheep, Dall,Dall Sheep,Ovis dalli
D013211 Staphylococcus aureus Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D054804 Cathelicidins Antimicrobial cationic peptides with a highly conserved amino terminal cathelin-like domain and a more variable carboxy terminal domain. They are initially synthesized as preproproteins and then cleaved. They are expressed in many tissues of humans and localized to EPITHELIAL CELLS. They kill nonviral pathogens by forming pores in membranes. ALL-38 Peptide,Antibacterial Peptide LL-37,Antimicrobial Peptide LL-37,Bac4 Protein, Bos taurus,CAP18 Lipopolysaccharide-Binding Protein,CATH-1 Protein,Cathelicidin,Cathelicidin 1,Cathelicidin Antimicrobial Peptide,Cathelicidin LL-37,Cathelicidin-1,Cathelin-Like Protein,Cathelin-Related Antimicrobial Peptide,LL-37 Antibacterial Peptide,LL-37 Peptide,Myeloid Cathelicidin 1 Protein, Equus caballus,Ropocamptide,eCATH-1,hCAP-18,CAP-18,CAP18,FA-LL-37,K9CATH,eCATH-1 protein, Equus caballus,ALL 38 Peptide,Antibacterial Peptide LL 37,Antibacterial Peptide, LL-37,Antimicrobial Peptide LL 37,Antimicrobial Peptide, Cathelin-Related,CAP 18,CAP18 Lipopolysaccharide Binding Protein,CATH 1 Protein,Cathelicidin LL 37,FA LL 37,LL 37 Antibacterial Peptide,LL 37 Peptide,LL-37, Antibacterial Peptide,LL-37, Antimicrobial Peptide,LL-37, Cathelicidin,Lipopolysaccharide-Binding Protein, CAP18,Peptide LL-37, Antibacterial,Peptide LL-37, Antimicrobial,Peptide, ALL-38,Peptide, Cathelin-Related Antimicrobial,Peptide, LL-37,Peptide, LL-37 Antibacterial,Protein, CAP18 Lipopolysaccharide-Binding,Protein, CATH-1,Protein, Cathelin-Like,eCATH 1,eCATH 1 protein, Equus caballus,hCAP 18

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