Proteomics, metabolomics, and immunomics on microparticles derived from human atherosclerotic plaques. 2009

Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
Cardiovascular Division, King's BHF Centre of Research Excellence, King's College London, London, United Kingdom. manuel.mayr@kcl.ac.uk

BACKGROUND Microparticles (MPs) with procoagulant activity are present in human atherosclerosis, but no detailed information is available on their composition. RESULTS To obtain insights into the role of MPs in atherogenesis, MP proteins were identified by tandem mass spectrometry, metabolite profiles were determined by high-resolution nuclear magnetic resonance spectroscopy, and antibody reactivity was assessed against combinatorial antigen libraries. Plaque MPs expressed surface antigens consistent with their leukocyte origin, including major histocompatibility complex classes I and II, and induced a dose-dependent stimulatory effect on T-cell proliferation. Notably, taurine, the most abundant free organic acid in human neutrophils, which scavenges myeloperoxidase-catalyzed free radicals, was highly enriched in plaque MPs. Moreover, fluorescent labeling of proteins on the MP surface suggested immunoglobulins to be trapped inside, which was confirmed by flow cytometry analysis on permeabilized and nonpermeabilized plaque MPs. Colabeling for CD14 and IgG established that more than 90% of the IgG containing MPs were CD14(+), indicating a macrophage origin. Screening against an antigen library revealed that the immunologic profiles of antibodies in MPs were similar to those found in plaques but differed profoundly from antibodies in plasma and unexpectedly, showed strong reactions with oligosaccharide antigens, in particular blood group antigen A. CONCLUSIONS This study provides the first evidence that immunoglobulins are present within MPs derived from plaque macrophages, that the portfolio of plaque antibodies is different from circulating antibodies in plasma, and that anticarbohydrate antibodies are retained in human atherosclerotic lesions.

UI MeSH Term Description Entries
D007074 Immunoglobulin G The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B. Gamma Globulin, 7S,IgG,IgG Antibody,Allerglobuline,IgG(T),IgG1,IgG2,IgG2A,IgG2B,IgG3,IgG4,Immunoglobulin GT,Polyglobin,7S Gamma Globulin,Antibody, IgG,GT, Immunoglobulin
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010316 Particle Size Relating to the size of solids. Particle Sizes,Size, Particle,Sizes, Particle
D005434 Flow Cytometry Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake. Cytofluorometry, Flow,Cytometry, Flow,Flow Microfluorimetry,Fluorescence-Activated Cell Sorting,Microfluorometry, Flow,Cell Sorting, Fluorescence-Activated,Cell Sortings, Fluorescence-Activated,Cytofluorometries, Flow,Cytometries, Flow,Flow Cytofluorometries,Flow Cytofluorometry,Flow Cytometries,Flow Microfluorometries,Flow Microfluorometry,Fluorescence Activated Cell Sorting,Fluorescence-Activated Cell Sortings,Microfluorimetry, Flow,Microfluorometries, Flow,Sorting, Fluorescence-Activated Cell,Sortings, Fluorescence-Activated Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D013654 Taurine A conditionally essential nutrient, important during mammalian development. It is present in milk but is isolated mostly from ox bile and strongly conjugates bile acids. Taufon,Tauphon,Taurine Hydrochloride,Taurine Zinc Salt (2:1),Taurine, Monopotassium Salt
D015180 Electrophoresis, Gel, Two-Dimensional Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels. Gel Electrophoresis, Two-Dimensional,Polyacrylamide Gel Electrophoresis, Two-Dimensional,2-D Gel Electrophoresis,2-D Polyacrylamide Gel Electrophoresis,2D Gel Electrophoresis,2D PAGE,2D Polyacrylamide Gel Electrophoresis,Electrophoresis, Gel, 2-D,Electrophoresis, Gel, 2D,Electrophoresis, Gel, Two Dimensional,Polyacrylamide Gel Electrophoresis, 2-D,Polyacrylamide Gel Electrophoresis, 2D,Two Dimensional Gel Electrophoresis,2 D Gel Electrophoresis,2 D Polyacrylamide Gel Electrophoresis,Electrophoresis, 2-D Gel,Electrophoresis, 2D Gel,Electrophoresis, Two-Dimensional Gel,Gel Electrophoresis, 2-D,Gel Electrophoresis, 2D,Gel Electrophoresis, Two Dimensional,PAGE, 2D,Polyacrylamide Gel Electrophoresis, 2 D,Polyacrylamide Gel Electrophoresis, Two Dimensional,Two-Dimensional Gel Electrophoresis
D050197 Atherosclerosis A thickening and loss of elasticity of the walls of ARTERIES that occurs with formation of ATHEROSCLEROTIC PLAQUES within the ARTERIAL INTIMA. Atherogenesis,Atherogeneses,Atheroscleroses

Related Publications

Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
December 2012, Thrombosis and haemostasis,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
February 2007, Journal of the American College of Cardiology,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
February 2011, Circulation research,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
February 1988, Atherosclerosis,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
November 1995, The American journal of pathology,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
January 2014, Thrombosis and haemostasis,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
October 2008, Journal of the American College of Cardiology,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
May 1995, The Journal of clinical investigation,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
February 1999, Apoptosis : an international journal on programmed cell death,
Manuel Mayr, and David Grainger, and Ursula Mayr, and Aurelie S Leroyer, and Guy Leseche, and Anissa Sidibe, and Olivier Herbin, and Xiaoke Yin, and Aldrin Gomes, and Bassetti Madhu, and John R Griffiths, and Qingbo Xu, and Alain Tedgui, and Chantal M Boulanger
July 2004, Thrombosis and haemostasis,
Copied contents to your clipboard!