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BACKGROUND Cannabinoid 1 receptors are identified in various tissues involved in the internal metabolism including adipose tissue and the endocannabinoid system is claimed to be overactive in the obese state. To study the potential involvement of cannabinoid receptor 1 in the endocannabinoid system over-activity in adipose tissue in the obese state, we investigated the cannabinoid receptor 1 levels in adipose tissue from different fat depots in lean and obese humans. METHODS The adipose tissue samples were analysed by Western blot and by RT-PCR. RESULTS Both the gene expression and the protein of cannabinoid receptor 1 were lower in subcutaneous abdominal adipose tissue from obese subjects as compared with lean subjects (P < 0.01 and P = 0.058). Moreover, in lean subjects, the level of cannabinoid receptor 1 was significantly higher in subcutaneous adipose tissue compared with visceral adipose tissue (P < 0.05) for both gene expression and protein. The level of cannabinoid receptor 1 was similar between the two depots in obese subjects. The expression of cannabinoid receptor 1 was higher in subcutaneous gluteal adipose tissue as compared with subcutaneous abdominal adipose tissue (P < 0.05). CONCLUSIONS We found in lean subjects, a robust lower level of cannabinoid receptor 1 in visceral adipose tissue compared with subcutaneous adipose tissue (both RNA and protein levels), but similar levels of cannabinoid receptor 1 between the two depots in obese subjects. Our present findings do not indicate that cannabinoid receptor 1 is directly involved in the endocannabinoid system over-activity in adipose tissue in obesity.
M F Bennetzen, and
T S Nielsen, and
S K Paulsen, and
J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
December 2011,
Diabetologia,
M F Bennetzen, and
T S Nielsen, and
S K Paulsen, and
J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
October 2012,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
M F Bennetzen, and
T S Nielsen, and
S K Paulsen, and
J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
January 2016,
Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association,
M F Bennetzen, and
T S Nielsen, and
S K Paulsen, and
J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
March 2019,
FASEB journal : official publication of the Federation of American Societies for Experimental Biology,
M F Bennetzen, and
T S Nielsen, and
S K Paulsen, and
J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
April 2002,
The Journal of pharmacology and experimental therapeutics,
M F Bennetzen, and
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J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
December 2015,
Journal of nuclear medicine : official publication, Society of Nuclear Medicine,
M F Bennetzen, and
T S Nielsen, and
S K Paulsen, and
J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
August 2013,
Metabolism: clinical and experimental,
M F Bennetzen, and
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J Bendix, and
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N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
June 2001,
International journal of obesity and related metabolic disorders : journal of the International Association for the Study of Obesity,
M F Bennetzen, and
T S Nielsen, and
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J Bendix, and
S Fisker, and
N Jessen, and
S Lund, and
B Richelsen, and
S B Pedersen
March 2019,
The Journal of steroid biochemistry and molecular biology,
M F Bennetzen, and
T S Nielsen, and
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J Bendix, and
S Fisker, and
N Jessen, and
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B Richelsen, and
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January 2018,
Magnetic resonance imaging,
