Simultaneous determination of quinolones for veterinary use by high-performance liquid chromatography with electrochemical detection. 2010

M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
Department of Analytical Chemistry, University of Extremadura, Avda. Elvas, S/N, 06071 Badajoz, Spain. maribelro@unex.es

A selective method based on high-performance liquid chromatography with electrochemical detection (HPLC-ECD) has been developed to enable simultaneous determination of three fluoroquinolones (FQs), namely danofloxacin (DANO), difloxacin (DIFLO) and sarafloxacin (SARA). The fluoroquinolones are separated on a Novapack C-18 column and detected in a high sensitivity amperometric cell at a potential of +0.8 V. Solid-phase extraction was used for the extraction of the analytes in real samples. The range of concentration examined varied from 10 to 150 ng g(-1) for danofloxacin, from 25 to 100 ng g(-1) for sarafloxacin and from 50 to 315 ng g(-1) for difloxacin, respectively. The method presents detection limits under 10 ng g(-1) and recoveries around 90% for the three analytes have been obtained in the experiments with fortified samples. This HPLC-ECD approach can be useful in the routine analysis of antibacterial residues being less expensive and less complicated than other more powerful tools as hyphenated techniques.

UI MeSH Term Description Entries
D012015 Reference Standards A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy. Standard Preparations,Standards, Reference,Preparations, Standard,Standardization,Standards,Preparation, Standard,Reference Standard,Standard Preparation,Standard, Reference
D012044 Regression Analysis Procedures for finding the mathematical function which best describes the relationship between a dependent variable and one or more independent variables. In linear regression (see LINEAR MODELS) the relationship is constrained to be a straight line and LEAST-SQUARES ANALYSIS is used to determine the best fit. In logistic regression (see LOGISTIC MODELS) the dependent variable is qualitative rather than continuously variable and LIKELIHOOD FUNCTIONS are used to find the best relationship. In multiple regression, the dependent variable is considered to depend on more than a single independent variable. Regression Diagnostics,Statistical Regression,Analysis, Regression,Analyses, Regression,Diagnostics, Regression,Regression Analyses,Regression, Statistical,Regressions, Statistical,Statistical Regressions
D002138 Calibration Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output. Calibrations
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D002939 Ciprofloxacin A broad-spectrum antimicrobial carboxyfluoroquinoline. Bay-09867,Ciprinol,Cipro,Ciprofloxacin Hydrochloride,Ciprofloxacin Hydrochloride Anhydrous,Ciprofloxacin Monohydrochloride Monohydrate,Anhydrous, Ciprofloxacin Hydrochloride,Bay 09867,Bay09867,Hydrochloride Anhydrous, Ciprofloxacin,Hydrochloride, Ciprofloxacin,Monohydrate, Ciprofloxacin Monohydrochloride,Monohydrochloride Monohydrate, Ciprofloxacin
D004566 Electrodes Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum. Anode,Anode Materials,Cathode,Cathode Materials,Anode Material,Anodes,Cathode Material,Cathodes,Electrode,Material, Anode,Material, Cathode
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012212 Rheology The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY. Flowmetry,Velocimetry,Velocimetries
D055664 Electrochemical Techniques The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues. Electrochemical Technics,Electrochemical Technic,Electrochemical Technique,Technic, Electrochemical,Technics, Electrochemical,Technique, Electrochemical,Techniques, Electrochemical

Related Publications

M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
June 2001, The Analyst,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
June 1988, Journal of chromatography,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
March 1980, Journal of chromatography,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
June 1997, Journal of pharmaceutical and biomedical analysis,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
April 1987, Journal of chromatography,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
February 1993, Lipids,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
July 1986, Journal of chromatography,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
January 2010, Journal of chromatography. A,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
April 2011, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
M I Rodríguez Cáceres, and A Guiberteau Cabanillas, and T Galeano Díaz, and M A Martínez Cañas
November 1984, Analytical biochemistry,
Copied contents to your clipboard!