Synaptic contribution of Ca2+-permeable and Ca2+-impermeable AMPA receptors on isolated carp retinal horizontal cells and their modulation by Zn2+. 2010

Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, China.

Ca(2+)-permeable and Ca(2+)-impermeable AMPA receptors are co-expressed on carp retinal horizontal cells. In the present study, we examined the synaptic contribution and Zn(2+) modulatory effect of these two AMPA receptor subtypes using whole-cell patch clamp technique. Specific Ca(2+)-permeable AMPA receptor antagonist (1-naphthyl acetyl spermine, NAS) and selective Ca(2+)-impermeable AMPA receptor blocker (pentobarbital, PB) were used to separate the glutamate-response in isolated H1 horizontal cell mediated by these two subtypes of AMPA receptors respectively. Application of 100 microM NAS substantially suppressed the current elicited by 3 mM glutamate and the remaining NAS-insensitive component was completely blocked by application of 100 microM PB. In addition, Zn(2+) had dual effects on Ca(2+)-permeable AMPA receptor-mediated current: at low concentration (10 microM), Zn(2+) potentiated the current, but at higher concentrations (100 and 1000 microM), Zn(2+) reduced the current in a dose-dependent manner. However, Zn(2+) (10, 100 and 1000 microM) failed to modulate the NAS-insensitive current mediated by Ca(2+)-impermeable AMPA receptors. Overall, our results suggest that Ca(2+)-permeable AMPA receptors contribute more to the cell's glutamate-response than Ca(2+)-impermeable AMPA receptors. Furthermore, Zn(2+) has dual effects on the Ca(2+)-permeable AMPA receptor activity without affecting Ca(2+)-impermeable AMPA receptors.

UI MeSH Term Description Entries
D007608 Kainic Acid (2S-(2 alpha,3 beta,4 beta))-2-Carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid. Ascaricide obtained from the red alga Digenea simplex. It is a potent excitatory amino acid agonist at some types of excitatory amino acid receptors and has been used to discriminate among receptor types. Like many excitatory amino acid agonists it can cause neurotoxicity and has been used experimentally for that purpose. Digenic Acid,Kainate,Acid, Digenic,Acid, Kainic
D008564 Membrane Potentials The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization). Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D010424 Pentobarbital A short-acting barbiturate that is effective as a sedative and hypnotic (but not as an anti-anxiety) agent and is usually given orally. It is prescribed more frequently for sleep induction than for sedation but, like similar agents, may lose its effectiveness by the second week of continued administration. (From AMA Drug Evaluations Annual, 1994, p236) Mebubarbital,Mebumal,Diabutal,Etaminal,Ethaminal,Nembutal,Pentobarbital Sodium,Pentobarbital, Monosodium Salt,Pentobarbitone,Sagatal,Monosodium Salt Pentobarbital
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002347 Carps Common name for a number of different species of fish in the family Cyprinidae. This includes, among others, the common carp, crucian carp, grass carp, and silver carp. Carassius carassius,Crucian Carp,Cyprinus,Grass Carp,Carp,Ctenopharyngodon idellus,Cyprinus carpio,Hypophthalmichthys molitrix,Koi Carp,Silver Carp,Carp, Crucian,Carp, Grass,Carp, Koi,Carp, Silver,Carps, Crucian,Carps, Grass,Carps, Silver,Crucian Carps,Grass Carps,Silver Carps
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013096 Spermine A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at all pH values. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure.
D013569 Synapses Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions. Synapse

Related Publications

Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
July 2010, Brain research,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
February 2005, Neuroreport,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
August 2006, The Journal of physiology,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
June 1998, Neuroscience research,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
June 1996, Nature,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
May 2021, The Journal of physiology,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
June 2006, Current opinion in neurobiology,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
March 2007, Trends in neurosciences,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
July 2014, PLoS biology,
Yan Sun, and Xiao-Dong Jiang, and Xue Liu, and Hai-Qing Gong, and Pei-Ji Liang
October 2012, The Journal of neuroscience : the official journal of the Society for Neuroscience,
Copied contents to your clipboard!