Cyclic AMP negatively regulates prodigiosin production by Serratia marcescens. 2010

Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
Charles T. Campbell Laboratory of Ophthalmic Microbiology, Department of Ophthalmology, University of Pittsburgh Eye Center, Pittsburgh, PA 15213, USA.

Many Serratia marcescens strains produce the red pigment prodigiosin, which has antimicrobial and anti-tumor properties. Previous reports suggest that cyclic AMP (cAMP) is a positive regulator of prodigiosin production. Supporting this model, the addition of glucose to growth medium inhibited pigment production in rich and minimal media. Unexpectedly, we observed highly elevated levels of prodigiosin production in isogenic strains with mutations in genes involved in cAMP production (cyaA and crr) and in cAMP-dependent transcriptional signaling (crp). Multicopy expression of the Escherichia coli cAMP-phosphodiesterase gene, cpdA, also conferred a striking increase in prodigiosin production. Exogenous cAMP decreased both pigment production and pigA-lacZ transcription in the wild-type (WT) strain, and pigA-lacZ transcription was significantly increased in a crp mutant relative to WT. Suppressor and epistasis analysis indicate that the hyperpigment phenotype was dependent upon pigment biosynthetic genes (pigA, pigB, pigC, pigD and pigM). These experiments establish cAMP as a negative regulator of prodigiosin production in S. marcescens.

UI MeSH Term Description Entries
D011353 Prodigiosin 4-Methoxy-5-((5-methyl-4-pentyl-2H-pyrrol-2-ylidene)methyl)- 2,2'-bi-1H-pyrrole. A toxic, bright red tripyrrole pigment from Serratia marcescens and others. It has antibacterial, anticoccidial, antimalarial, and antifungal activities, but is used mainly as a biochemical tool.
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D005947 Glucose A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement. Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D000242 Cyclic AMP An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenosine Cyclic 3',5'-Monophosphate,Adenosine Cyclic 3,5 Monophosphate,Adenosine Cyclic Monophosphate,Adenosine Cyclic-3',5'-Monophosphate,Cyclic AMP, (R)-Isomer,Cyclic AMP, Disodium Salt,Cyclic AMP, Monoammonium Salt,Cyclic AMP, Monopotassium Salt,Cyclic AMP, Monosodium Salt,Cyclic AMP, Sodium Salt,3',5'-Monophosphate, Adenosine Cyclic,AMP, Cyclic,Adenosine Cyclic 3',5' Monophosphate,Cyclic 3',5'-Monophosphate, Adenosine,Cyclic Monophosphate, Adenosine,Cyclic-3',5'-Monophosphate, Adenosine,Monophosphate, Adenosine Cyclic
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D012706 Serratia marcescens A species of gram-negative, facultatively anaerobic, rod-shaped bacteria found in soil, water, food, and clinical specimens. It is a prominent opportunistic pathogen for hospitalized patients.
D017353 Gene Deletion A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus. Deletion, Gene,Deletions, Gene,Gene Deletions

Related Publications

Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
May 2014, Archives of microbiology,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
November 1954, Applied microbiology,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
July 2014, Applied biochemistry and biotechnology,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
August 1971, Journal of general microbiology,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
February 1963, Nature,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
May 1968, Nature,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
January 2023, Frontiers in microbiology,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
May 2024, Biochemical and biophysical research communications,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
April 1976, Experientia,
Eric J Kalivoda, and Nicholas A Stella, and Marissa A Aston, and James E Fender, and Paul P Thompson, and Regis P Kowalski, and Robert M Q Shanks
January 2022, Frontiers in microbiology,
Copied contents to your clipboard!