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Identification of binding proteins of fusarielin A as actin and tubulin. 2010
Tomomi Noguchi-Yachide, and
Kosuke Dodo, and
Hiroshi Aoyama, and
Haruka Fujimoto, and
Masatoshi Hori, and
Yuichi Hashimoto, and
Hisayoshi Kobayashi
Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo, Japan.
Fusarielin A (FSA), isolated from culture of Fusarium sp. K432, has anti-fungal/anti-angiogenic activity. Based on our previous structure-activity relationship studies, we prepared FSA-immobilized affinity gel. The results of affinity chromatography with this gel indicated that tubulin and actin are FSA-binding proteins. The binding affinities of tubulin and actin for FSA were estimated by the use of surface plasmon resonance spectroscopy.
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