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We examined the presence of cathepsins B, H, and L in bronchoalveolar epithelial cells, including alveolar macrophages, and in bronchoalveolar lavage fluid (BALF), using immunocytochemistry and immunoblotting. By light and electron microscopy, immunoreactivity for cathepsins B, H, and L was detected in lysosomes of ciliated and non-ciliated epithelial cells of bronchi and bronchioles, and in macrophages. Immunodeposits for cathepsin H only were demonstrated in lamellar bodies of Type II alveolar epithelial cells, suggesting the cosecretion of surfactants and cathepsin H from the cells into the alveolar space. By immunoblotting, cathepsins B and H were found to be present in BALF. To further investigate the origin of these enzymes in BALF, alveolar macrophages obtained from BALF were cultured for 6 hr in a serum-free medium. Immunoblotting revealed that protein bands corresponding to the pro-form and mature form of cathepsin B and the mature form of cathepsin H were present in the culture medium. From these results, the presence of cathepsins B and H in BALF can be explained by the fact that cathepsin B is secreted from alveolar macrophages and cathepsin H is secreted mainly with surfactants from Type II cells and also from macrophages.
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
November 1998,
The European respiratory journal,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
January 1993,
Cancer detection and prevention,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
September 1988,
The Journal of laboratory and clinical medicine,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
January 1993,
Human & experimental toxicology,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
January 1990,
Free radical biology & medicine,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
September 2007,
Annales de pathologie,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
November 1989,
Nihon Kyobu Shikkan Gakkai zasshi,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
April 2003,
Pneumologie (Stuttgart, Germany),
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
September 2009,
Proteomics. Clinical applications,
Y Ishii, and
Y Hashizume, and
T Watanabe, and
S Waguri, and
N Sato, and
M Yamamoto, and
S Hasegawa, and
E Kominami, and
Y Uchiyama
August 2022,
Cancer research,
