Effect of probiotic bacterial strains of Lactobacillus, Bifidobacterium, and Enterococcus on enteroaggregative Escherichia coli. 2010

Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
Department of Emergency and Critical Care Medicine, Nippon Medical School, Tokyo, Japan.

The effects of nine probiotic strains of Lactobacillus, Bifidobacterium, and Enterococcus on the growth, adhesion activity, and biofilm formation of enteroaggregative Escherichia coli (EAggEC) were examined. The culture supernatant of the E. faecium strain, with or without pH adjustment to a neutral pH, had a strong bactericidal effect on EAggEC, including induction of membrane damage and cell lysis. Supernatants of the L. casei ss. casei and L. casei ss. rhamnosus strains also had a bactericidal effect on EAggEC, but this activity was abolished by pH adjustment to a neutral pH. No inhibitory effect of the culture supernatants of Bifidobacterium or E. faecalis strains was detected. Adhesion of EAggEC to intestinal epithelial cells was not inhibited by the bacterial strains tested. Two strains of L. casei enhanced EAggEC biofilm formation, which was characterized by increased bacterial proliferation. These results suggest that the three different bacterial species; Lactobacillus, Bifidobacterium, and Enterococcus, have different effects on EAggEC, and that further analysis is required for the practical use of these bacteria as probiotics against EAggEC infection.

UI MeSH Term Description Entries
D007422 Intestines The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE. Intestine
D007778 Lactobacillus A genus of gram-positive, microaerophilic, rod-shaped bacteria occurring widely in nature. Its species are also part of the many normal flora of the mouth, intestinal tract, and vagina of many mammals, including humans. Lactobacillus species are homofermentative and ferment a broad spectrum of carbohydrates often host-adapted but do not ferment PENTOSES. Most members were previously assigned to the Lactobacillus delbrueckii group. Pathogenicity from this genus is rare.
D004847 Epithelial Cells Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells. Adenomatous Epithelial Cells,Columnar Glandular Epithelial Cells,Cuboidal Glandular Epithelial Cells,Glandular Epithelial Cells,Squamous Cells,Squamous Epithelial Cells,Transitional Epithelial Cells,Adenomatous Epithelial Cell,Cell, Adenomatous Epithelial,Cell, Epithelial,Cell, Glandular Epithelial,Cell, Squamous,Cell, Squamous Epithelial,Cell, Transitional Epithelial,Cells, Adenomatous Epithelial,Cells, Epithelial,Cells, Glandular Epithelial,Cells, Squamous,Cells, Squamous Epithelial,Cells, Transitional Epithelial,Epithelial Cell,Epithelial Cell, Adenomatous,Epithelial Cell, Glandular,Epithelial Cell, Squamous,Epithelial Cell, Transitional,Epithelial Cells, Adenomatous,Epithelial Cells, Glandular,Epithelial Cells, Squamous,Epithelial Cells, Transitional,Glandular Epithelial Cell,Squamous Cell,Squamous Epithelial Cell,Transitional Epithelial Cell
D004926 Escherichia coli A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc. Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000898 Antibiosis A natural association between organisms that is detrimental to at least one of them. This often refers to the production of chemicals by one microorganism that is harmful to another. Bacterial Interference,Microbial Antagonism,Interference, Bacterial,Antagonism, Microbial,Antagonisms, Microbial,Antibioses,Bacterial Interferences,Interferences, Bacterial,Microbial Antagonisms
D001422 Bacterial Adhesion Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity. Adhesion, Bacterial,Adhesions, Bacterial,Bacterial Adhesions
D001644 Bifidobacterium A rod-shaped, gram-positive, non-acid-fast, non-spore-forming, non-motile bacterium that is a genus of the family Bifidobacteriaceae, order Bifidobacteriales, class ACTINOBACTERIA. It inhabits the intestines and feces of humans as well as the human vagina.
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D016983 Enterococcus A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.

Related Publications

Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
August 2009, Archives of microbiology,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
January 2020, Voprosy pitaniia,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
February 2005, International journal of food microbiology,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
January 1996, Zhurnal mikrobiologii, epidemiologii i immunobiologii,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
July 2015, FEMS microbiology letters,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
September 2002, Journal of clinical microbiology,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
January 2019, Frontiers in nutrition,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
January 2021, Arquivos de gastroenterologia,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
April 2007, Applied microbiology and biotechnology,
Yoshibumi Miyazaki, and Shigeru Kamiya, and Tomoko Hanawa, and Minoru Fukuda, and Hayato Kawakami, and Hidemi Takahashi, and Hiroyuki Yokota
January 2008, The ISME journal,
Copied contents to your clipboard!