Biomaterial Database

A novel visible spectrophotometric method for the determination of ethamsylate in pharmaceutical preparations and biological samples. 2010

Meiyun Zhang, and Yan Zhang, and Quanmin Li

College of Chemistry and Environmental Science, Henan Normal University, Henan Key Laboratory for Environmental Pollution Control, Xinxiang, Henan, PR China.

A highly sensitive visible spectrophotometric method has been developed to determine ethamsylate in this paper, which is based on using Cu(II) as spectroscopic probe reagent. The study indicates that in the presence of SCN(-) and KNO(3), Cu(II) is reduced to Cu(I) by ethamsylate at pH 5.0, and the in situ formed Cu(I) reacts with SCN(-) to form into the white emulsion CuSCN that could be stayed upon the surface of water. According to the amount of residual Cu(II), the amount of ethamsylate can be indirectly determined. Under the optimal conditions, Beer's law is applicable in the range of 0.2-9.0 microg/mL (7.60x10(-7)-3.42x10(-5)mol/L) for aqueous standard solution of ethamsylate with linear correlation coefficient of 0.9998. The detection limit and relative standard deviation are 0.12 microg/mL and 1.5%, respectively. And the molar absorption coefficient of the indirect determination of ethamsylate is 1.0x10(5)L/mol cm. The method is successfully applied to determine ethamsylate in pharmaceutical preparations and biological samples.

UI	MeSH Term	Description	Entries
D001826	Body Fluids	Liquid components of living organisms.	Body Fluid,Fluid, Body,Fluids, Body
D003300	Copper	A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.	Copper-63,Copper 63
D004364	Pharmaceutical Preparations	Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form.	Drug,Drugs,Pharmaceutical,Pharmaceutical Preparation,Pharmaceutical Product,Pharmaceutic Preparations,Pharmaceutical Products,Pharmaceuticals,Preparations, Pharmaceutical,Preparation, Pharmaceutical,Preparations, Pharmaceutic,Product, Pharmaceutical,Products, Pharmaceutical
D004396	Coloring Agents	Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.	Coloring Agent,Dye,Dyes,Organic Pigment,Stain,Stains,Tissue Stain,Tissue Stains,Organic Pigments,Pigments, Inorganic,Agent, Coloring,Inorganic Pigments,Pigment, Organic,Pigments, Organic,Stain, Tissue,Stains, Tissue
D004979	Ethamsylate	Benzenesulfonate derivative used as a systemic hemostatic.	Cyclonamine,Altodor,Dicinone,Dicynene,Dicynone,Etamsylate,Hemo 141
D006490	Hemostatics	Agents acting to arrest the flow of blood. Absorbable hemostatics arrest bleeding either by the formation of an artificial clot or by providing a mechanical matrix that facilitates clotting when applied directly to the bleeding surface. These agents function more at the capillary level and are not effective at stemming arterial or venous bleeding under any significant intravascular pressure.	Antihemorrhagic,Hemostatic,Antihemorrhagics
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D013861	Thiocyanates	Organic derivatives of thiocyanic acid which contain the general formula R-SCN.	Rhodanate,Rhodanates
D015394	Molecular Structure	The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.	Structure, Molecular,Molecular Structures,Structures, Molecular
D057230	Limit of Detection	Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.	Limits of Detection,Detection Limit,Detection Limits