Electrogenerated chemiluminescence of blue emitting ZnSe quantum dots and its biosensing for hydrogen peroxide. 2010

Xiaofeng Hu, and Heyou Han, and Lijuan Hua, and Zonghai Sheng
College of Science, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei 430070, PR China.

A novel method for the detection of hydrogen peroxide (H(2)O(2)) has been developed, based on electrogenerated chemiluminescence (ECL) of blue emitting ZnSe quantum dots (QDs) in aqueous solution. The weak ECL emission of ZnSe QDs was generated when the electrode potential was scanned from 0.0 to -1.4V (versus Ag/AgCl), and the ECL intensity was greatly enhanced in the presence of H(2)O(2) or dissolved oxygen. Under the optimal conditions, the dynamic range is from 6.10 x 10(-7) to 3.10 x 10(-4)M H(2)O(2), and the detection limit (at S/N=3) is 2.00 x 10(-7)M. The relative standard deviation (at 5.00 x 10(-5)M of H(2)O(2)) is 2.70% (n=11). A possible reaction mechanism of ECL of ZnSe QDs was also investigated. As a novel biosensor, it was used to detect H(2)O(2) in the pig kidney (pk-15) cell, vero cell and mineral water, respectively. Moreover, the proposed biosensor showed a good reproducibility and potential application in real samples.

UI MeSH Term Description Entries
D008163 Luminescent Measurements Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE. Bioluminescence Measurements,Bioluminescent Assays,Bioluminescent Measurements,Chemiluminescence Measurements,Chemiluminescent Assays,Chemiluminescent Measurements,Chemoluminescence Measurements,Luminescence Measurements,Luminescent Assays,Luminescent Techniques,Phosphorescence Measurements,Phosphorescent Assays,Phosphorescent Measurements,Assay, Bioluminescent,Assay, Chemiluminescent,Assay, Luminescent,Assay, Phosphorescent,Assays, Bioluminescent,Assays, Chemiluminescent,Assays, Luminescent,Assays, Phosphorescent,Bioluminescence Measurement,Bioluminescent Assay,Bioluminescent Measurement,Chemiluminescence Measurement,Chemiluminescent Assay,Chemiluminescent Measurement,Chemoluminescence Measurement,Luminescence Measurement,Luminescent Assay,Luminescent Measurement,Luminescent Technique,Measurement, Bioluminescence,Measurement, Bioluminescent,Measurement, Chemiluminescence,Measurement, Chemiluminescent,Measurement, Chemoluminescence,Measurement, Luminescence,Measurement, Luminescent,Measurement, Phosphorescence,Measurement, Phosphorescent,Measurements, Bioluminescence,Measurements, Bioluminescent,Measurements, Chemiluminescence,Measurements, Chemiluminescent,Measurements, Chemoluminescence,Measurements, Luminescence,Measurements, Luminescent,Measurements, Phosphorescence,Measurements, Phosphorescent,Phosphorescence Measurement,Phosphorescent Assay,Phosphorescent Measurement,Technique, Luminescent,Techniques, Luminescent
D003116 Color The visually perceived property of objects created by absorption or reflection of specific wavelengths of light. Colors
D003217 Conductometry Determination of the quantity of a material present in a mixture by measurement of its effect on the electrical conductivity of the mixture. (Webster, 3d ed) Titration, Conductometric,Conductometric Titration,Conductometric Titrations,Titrations, Conductometric
D004581 Electronics The study, control, and application of the conduction of ELECTRICITY through gases or vacuum, or through semiconducting or conducting materials. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed) Electronic
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D006861 Hydrogen Peroxide A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials. Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D015374 Biosensing Techniques Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal. Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D045663 Quantum Dots Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES. Nanocrystals, Semiconductor,Semiconductor Nanocrystals,Semiconductor Nanoparticles,Dot, Quantum,Dots, Quantum,Nanocrystal, Semiconductor,Nanoparticle, Semiconductor,Nanoparticles, Semiconductor,Quantum Dot,Semiconductor Nanocrystal,Semiconductor Nanoparticle
D017967 Zinc Compounds Inorganic compounds that contain zinc as an integral part of the molecule. Compounds, Zinc
D018036 Selenium Compounds Inorganic compounds that contain selenium as an integral part of the molecule. Compounds, Selenium

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