Property of I(K,)(n) in inner hair cells isolated from guinea-pig cochlea. 2010

Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
Department of Otolaryngology, Graduate School of Medical Sciences, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higasi-Ku, Fukuoka 812-8582, Japan. kimitaka@qent.med.kyushu-u.ac.jp

One of the potassium currents, I(K,)(n), is already activated at the resting potential of the cell and thus determines the membrane potential. KCNQ4 channel has been identified as the molecular correlate of I(K,)(n). In the present study, we measured I(K,)(n) in acutely isolated IHCs of guinea-pig cochlea using the whole-cell voltage-clamp techniques, and investigated the properties of the currents. I(K,)(n) was 70% activated around the resting potential of -60 mV and deactivated on hyperpolarization. I(K,)(n) was blocked by the KCNQ-channel blockers, linopirdine (100 microM) and XE991 (10 microM), but was insensitive to both I(K,f) blocker, tetraethylammonium (TEA), and I(K,s) blocker, 4-aminopyridine (4-AP). There was no significant difference in the size of I(K,)(n) between the apical and basal turn IHCs.

UI MeSH Term Description Entries
D007211 Indoles Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
D008564 Membrane Potentials The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization). Resting Potentials,Transmembrane Potentials,Delta Psi,Resting Membrane Potential,Transmembrane Electrical Potential Difference,Transmembrane Potential Difference,Difference, Transmembrane Potential,Differences, Transmembrane Potential,Membrane Potential,Membrane Potential, Resting,Membrane Potentials, Resting,Potential Difference, Transmembrane,Potential Differences, Transmembrane,Potential, Membrane,Potential, Resting,Potential, Transmembrane,Potentials, Membrane,Potentials, Resting,Potentials, Transmembrane,Resting Membrane Potentials,Resting Potential,Transmembrane Potential,Transmembrane Potential Differences
D011725 Pyridines Compounds with a six membered aromatic ring containing NITROGEN. The saturated version is PIPERIDINES.
D003051 Cochlea The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH. Cochleas
D006168 Guinea Pigs A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research. Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006199 Hair Cells, Auditory, Inner Auditory sensory cells of organ of Corti, usually placed in one row medially to the core of spongy bone (the modiolus). Inner hair cells are in fewer numbers than the OUTER AUDITORY HAIR CELLS, and their STEREOCILIA are approximately twice as thick as those of the outer hair cells. Auditory Hair Cell, Inner,Auditory Hair Cells, Inner,Cochlear Inner Hair Cell,Cochlear Inner Hair Cells,Hair Cell, Auditory, Inner,Inner Auditory Hair Cell,Inner Auditory Hair Cells,Inner Hair Cells,Cell, Inner Hair,Cells, Inner Hair,Hair Cell, Inner,Hair Cells, Inner,Inner Hair Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015761 4-Aminopyridine One of the POTASSIUM CHANNEL BLOCKERS with secondary effect on calcium currents which is used mainly as a research tool and to characterize channel subtypes. 4-Aminopyridine Sustained Release,Dalfampridine,Fampridine-SR,Pymadine,VMI-103,4 Aminopyridine,4 Aminopyridine Sustained Release,Fampridine SR,Sustained Release, 4-Aminopyridine,VMI 103,VMI103
D051656 KCNQ Potassium Channels A family of delayed rectifier voltage-gated potassium channels that share homology with their founding member, KCNQ1 PROTEIN. KCNQ potassium channels have been implicated in a variety of diseases including LONG QT SYNDROME; DEAFNESS; and EPILEPSY. KvLQT Potassium Channels,Potassium Channels, KCNQ,Potassium Channels, KvLQT
D018408 Patch-Clamp Techniques An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used. Patch Clamp Technique,Patch-Clamp Technic,Patch-Clamp Technique,Voltage-Clamp Technic,Voltage-Clamp Technique,Voltage-Clamp Techniques,Whole-Cell Recording,Patch-Clamp Technics,Voltage-Clamp Technics,Clamp Technique, Patch,Clamp Techniques, Patch,Patch Clamp Technic,Patch Clamp Technics,Patch Clamp Techniques,Recording, Whole-Cell,Recordings, Whole-Cell,Technic, Patch-Clamp,Technic, Voltage-Clamp,Technics, Patch-Clamp,Technics, Voltage-Clamp,Technique, Patch Clamp,Technique, Patch-Clamp,Technique, Voltage-Clamp,Techniques, Patch Clamp,Techniques, Patch-Clamp,Techniques, Voltage-Clamp,Voltage Clamp Technic,Voltage Clamp Technics,Voltage Clamp Technique,Voltage Clamp Techniques,Whole Cell Recording,Whole-Cell Recordings

Related Publications

Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
February 1990, The Journal of physiology,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
February 2017, Auris, nasus, larynx,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
October 2015, Auris, nasus, larynx,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
January 2013, Acta neurobiologiae experimentalis,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
October 1999, HNO,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
July 1984, Hearing research,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
April 2001, Zhonghua er bi yan hou ke za zhi,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
March 1992, The Journal of physiology,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
April 1991, Neuroscience letters,
Takashi Kimitsuki, and Noritaka Komune, and Teppei Noda, and Kazutaka Takaiwa, and Mitsuru Ohashi, and Shizuo Komune
November 2001, Hearing research,
Copied contents to your clipboard!