Methylenetetrahydrofolate reductase gene polymorphisms and risk of acute lymphoblastic leukemia in children. 2010

M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
Department of Biochemistry, Kidwai Memorial Institute of Oncology, Dr. M. H. Marigowda Road, Bangalore-560 029, India.

BACKGROUND Methylenetetrahydrofolate reductase (MTHFR) is a critical enzyme in folate metabolism and is involved in DNA synthesis, DNA repair and DNA methylation. Genetic polymorphisms of this enzyme have been shown to impact several diseases, including cancer. Leukemias are malignancies arising from rapidly proliferating hematopoietic cells having great requirement of DNA synthesis. This case-control study was undertaken to analyze the association of the MTHFR gene polymorphisms 677 C"T and 1298 A"C and the risk of acute lymphoblastic leukemia in children. METHODS Eighty-six patients aged below 15 years with a confirmed diagnosis of acute lymphoblastic leukemia (ALL) and 99 matched controls were taken for this study. Analysis of the polymorphisms was done using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. RESULTS Frequency of MTHFR 677 CC and CT were 85.9% and 14.1% in the controls, and 84.9% and 15.1% in the cases. The 'T' allele frequency was 7% and 7.5% in cases and controls respectively. The frequency of MTHFR 1298 AA, AC, and CC were 28.3%, 55.6% and 16.1% for controls and 23.3%, 59.3% and 17.4% for cases respectively. The 'C' allele frequency for 1298 A-->C was 43.9% and 47% respectively for controls and cases. The odds ratio (OR) for C677T was 1.08 (95% CI 0.48-2.45, p = 0.851) and OR for A1298C was 1.29 (95% CI 0.65-2.29, p = 0.46) and OR for 1298 CC was 1.31 (95% CI 0.53-3.26, p = 0.56). The OR for the combined heterozygous status (677 CT and 1298 AC) was 1.94 (95% CI 0.58-6.52, p = 0.286). CONCLUSIONS The prevalence of 'T' allele for 677 MTHFR polymorphism was low in the population studied. There was no association between MTHFR 677 C-->T and 1298 A-->C gene polymorphisms and risk of ALL, which may be due to the small sample size.

UI MeSH Term Description Entries
D008297 Male Males
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D012307 Risk Factors An aspect of personal behavior or lifestyle, environmental exposure, inborn or inherited characteristic, which, based on epidemiological evidence, is known to be associated with a health-related condition considered important to prevent. Health Correlates,Risk Factor Scores,Risk Scores,Social Risk Factors,Population at Risk,Populations at Risk,Correlates, Health,Factor, Risk,Factor, Social Risk,Factors, Social Risk,Risk Factor,Risk Factor Score,Risk Factor, Social,Risk Factors, Social,Risk Score,Score, Risk,Score, Risk Factor,Social Risk Factor
D016022 Case-Control Studies Comparisons that start with the identification of persons with the disease or outcome of interest and a control (comparison, referent) group without the disease or outcome of interest. The relationship of an attribute is examined by comparing both groups with regard to the frequency or levels of outcome over time. Case-Base Studies,Case-Comparison Studies,Case-Referent Studies,Matched Case-Control Studies,Nested Case-Control Studies,Case Control Studies,Case-Compeer Studies,Case-Referrent Studies,Case Base Studies,Case Comparison Studies,Case Control Study,Case Referent Studies,Case Referrent Studies,Case-Comparison Study,Case-Control Studies, Matched,Case-Control Studies, Nested,Case-Control Study,Case-Control Study, Matched,Case-Control Study, Nested,Case-Referent Study,Case-Referrent Study,Matched Case Control Studies,Matched Case-Control Study,Nested Case Control Studies,Nested Case-Control Study,Studies, Case Control,Studies, Case-Base,Studies, Case-Comparison,Studies, Case-Compeer,Studies, Case-Control,Studies, Case-Referent,Studies, Case-Referrent,Studies, Matched Case-Control,Studies, Nested Case-Control,Study, Case Control,Study, Case-Comparison,Study, Case-Control,Study, Case-Referent,Study, Case-Referrent,Study, Matched Case-Control,Study, Nested Case-Control
D016133 Polymerase Chain Reaction In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. Anchored PCR,Inverse PCR,Nested PCR,PCR,Anchored Polymerase Chain Reaction,Inverse Polymerase Chain Reaction,Nested Polymerase Chain Reaction,PCR, Anchored,PCR, Inverse,PCR, Nested,Polymerase Chain Reactions,Reaction, Polymerase Chain,Reactions, Polymerase Chain
D042965 Methylenetetrahydrofolate Reductase (NADPH2) A flavoprotein amine oxidoreductase that catalyzes the reversible conversion of 5-methyltetrahydrofolate to 5,10-methylenetetrahydrofolate. This enzyme was formerly classified as EC 1.1.1.171. Methylene-THF Reductase (NADPH),5,10-Methylenetetrahydrofolate Reductase (NADPH),Methylene Tetrahydrofolate Reductase,Methylenetetrahydrofolate Reductase,Methylenetetrahydrofolate Reductase (NADPH),Tetrahydrofolate Reductase, Methylene

Related Publications

M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
May 2010, Journal of pediatric hematology/oncology,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
October 2006, Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
March 2010, Cancer science,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
May 2010, Leukemia & lymphoma,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
August 2008, Pediatric blood & cancer,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
January 2006, European journal of epidemiology,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
March 2005, Cancer research,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
April 2002, Leukemia,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
January 2006, European journal of epidemiology,
M N Sadananda Adiga, and S Chandy, and N Ramachandra, and L Appaji, and B S Aruna Kumari, and G Ramaswamy, and H S Savithri, and L Krishnamoorthy
October 2011, Leukemia & lymphoma,
Copied contents to your clipboard!