-transparent.png){kind=logo}
A sequential immunoaffinity chromatography procedure was developed to isolate from whole normolipidemic human plasma a subpopulation of apoB containing particles (Lp-B) which is virtually free of non apoB protein. The absence of non apoB protein in Lp-B was assessed by enzyme immunoassay against apolipoproteins A-I, A-II, A-IV, E, C-III and (a). Electron microscopy and fractionation of the isolated particles by gel filtration demonstrated that these particles were heterogeneous in size. However, most of them had diameters between 18 and 26 nm. These particles were found to be rich in cholesterol (molar ratio cholesterol/apoB = 2246 +/- 995) poor in triacylglycerol (molar ratio triacylglycerol/apoB = 555 +/- 518) and had a phospholipids/apoB molar ratio of 713 +/- 348. Most of the cholesterol was esterified (66% +/- 5%). Lp-B particles bound to the apoB, E receptor of HeLa cells with a lower affinity than LDL prepared by ultracentrifugation (1.030 kg/l less than d less than 1.053 kg/l). (KD = 18.9 vs 10.5 nmol/l).
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
June 1992,
The Biochemical journal,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
March 1986,
Biochimica et biophysica acta,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
January 2024,
Journal of the American College of Cardiology,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
August 2006,
Journal of lipid research,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
April 1982,
Atherosclerosis,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
April 1995,
The Biochemical journal,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
September 1975,
Metabolism: clinical and experimental,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
October 1986,
Biochemistry and cell biology = Biochimie et biologie cellulaire,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
December 1973,
FEBS letters,
J M Bard, and
L Candelier, and
G Agnani, and
V Clavey, and
G Torpier, and
A Steinmetz, and
J C Fruchart
April 1986,
Rinsho byori. The Japanese journal of clinical pathology,
