Biomaterial Database

Protective role of Annona squamosa linn bark extracts in DMBA induced genotoxicity. 2008

K Suresh, and S Manoharn, and D Blessy

Department of Biochemistry and Biotechnology, Faculty of science, Annamalai University, Tamil Nadu, India. suraj_cks@yahoo.co.in

BACKGROUND There has been no dearth of scientific literature that genotoxic agents have a causative effect in the pathogenesis of carcinomas. 7,12 dimethylbenz(a)anthracene (DMBA), which is a potent organ specific carcinogen, has been found to produce mutagenic effects on cells. Annona squamosa has a long history in traditional Indian medicine for being used to treat several diseases including cancer. OBJECTIVE Aim of the present study was to investigate the protective role of Annona squamosa bark extracts in DMBA induced genotoxicity in golden Syrian hamsters. METHODS Genotoxicity was induced in golden Syrian hamsters by single intraperitoneal injection of DMBA (30 mg/kg body weight). The antigenotoxic effect of aqueous and ethanolic bark extracts of Annona squamosa was assessed by determining the frequency of micronucleated polychromatic erythrocytes (MnPCEs) and chromosomal aberrations. RESULTS The frequency of MnPCEs and chromosomal aberrations in bone marrow were higher in DMBA treated animals as compared to control animals. Oral administration of aqueous and ethanolic bark extracts significantly reduced the frequency of MnPCEs and chromosomal aberration in DMBA treated hamsters. CONCLUSIONS Although both extracts have shown antigenotoxic effect, the effect of ethanolic extract was found to be more prominent than the aqueous extract. The present study thus demonstrates the antigenotoxic effect of Annona squamosa bark extracts in DMBA induced genotoxicity in golden Syrian hamsters.

UI	MeSH Term	Description	Entries
D007274	Injections, Intraperitoneal	Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall.	Intraperitoneal Injections,Injection, Intraperitoneal,Intraperitoneal Injection
D008297	Male		Males
D008517	Phytotherapy	Use of plants or herbs to treat diseases or to alleviate pain.	Herb Therapy,Herbal Therapy
D008647	Mesocricetus	A genus in the order Rodentia and family Cricetidae. One species, Mesocricetus auratus or golden hamster is widely used in biomedical research.	Hamsters, Golden,Hamsters, Golden Syrian,Hamsters, Syrian,Mesocricetus auratus,Syrian Golden Hamster,Syrian Hamster,Golden Hamster,Golden Hamster, Syrian,Golden Hamsters,Golden Syrian Hamsters,Hamster, Golden,Hamster, Syrian,Hamster, Syrian Golden,Syrian Hamsters
D010936	Plant Extracts	Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard.	Herbal Medicines,Plant Extract,Extract, Plant,Extracts, Plant,Medicines, Herbal
D002273	Carcinogens	Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.	Carcinogen,Oncogen,Oncogens,Tumor Initiator,Tumor Initiators,Tumor Promoter,Tumor Promoters,Initiator, Tumor,Initiators, Tumor,Promoter, Tumor,Promoters, Tumor
D002869	Chromosome Aberrations	Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.	Autosome Abnormalities,Cytogenetic Aberrations,Abnormalities, Autosome,Abnormalities, Chromosomal,Abnormalities, Chromosome,Chromosomal Aberrations,Chromosome Abnormalities,Cytogenetic Abnormalities,Aberration, Chromosomal,Aberration, Chromosome,Aberration, Cytogenetic,Aberrations, Chromosomal,Aberrations, Chromosome,Aberrations, Cytogenetic,Abnormalities, Cytogenetic,Abnormality, Autosome,Abnormality, Chromosomal,Abnormality, Chromosome,Abnormality, Cytogenetic,Autosome Abnormality,Chromosomal Aberration,Chromosomal Abnormalities,Chromosomal Abnormality,Chromosome Aberration,Chromosome Abnormality,Cytogenetic Aberration,Cytogenetic Abnormality
D004195	Disease Models, Animal	Naturally-occurring or experimentally-induced animal diseases with pathological processes analogous to human diseases.	Animal Disease Model,Animal Disease Models,Disease Model, Animal
D004249	DNA Damage	Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.	DNA Injury,DNA Lesion,DNA Lesions,Genotoxic Stress,Stress, Genotoxic,Injury, DNA,DNA Injuries
D006224	Cricetinae	A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.	Cricetus,Hamsters,Hamster