Biomaterial Database

[Expression and character of measles virus hemagglutinin gene]. 2009

Liang Liang, and Yin Chen, and Yi-Yu Lu

The College of Life Science, Zhejiang Sic-Tech University, Hangzhou 310018, Zhejiang, China.

OBJECTIVE To express the hemagglutinin gene of measles virus (MV) in Escherichia col iexpression system, and apply the recombinant protein for detection of MV. METHODS Measles virus genomic RNA was extracted from the strain MVi/Zhejiang. CHN/7.05/4, the interest H gene was amplified by RT-PCR and subcloned into the pET28a (+) vector, the recombinant plasmid was transformed into E. coli BL21(DE3) cells and induced with IPTG. After the expression of the recombinant protein, it was analyzed with SDS-PAGE, while Western blotting was performed to determine the specific antigenicity of the target protein. Then, the recombinant protein purified with the Ni-NTA column was applied for the ELISA detection. RESULTS The interest gene amplified by RT-PCR was 1700bp, and there was no mutation by sequencing. The interest protein was expressed in inclusion in Escherichia coli expression system, and showed the high antigenicity in Western blotting. CONCLUSIONS The interest protein was successfully expressed in prokaryotic system, and can be used as the antigen in measles virus detection after it was purified.

UI	MeSH Term	Description	Entries
D008459	Measles virus	The type species of MORBILLIVIRUS and the cause of the highly infectious human disease MEASLES, which affects mostly children.	Edmonston virus
D008970	Molecular Weight	The sum of the weight of all the atoms in a molecule.	Molecular Weights,Weight, Molecular,Weights, Molecular
D011994	Recombinant Proteins	Proteins prepared by recombinant DNA technology.	Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D003001	Cloning, Molecular	The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.	Molecular Cloning
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D006388	Hemagglutinins	Agents that cause agglutination of red blood cells. They include antibodies, blood group antigens, lectins, autoimmune factors, bacterial, viral, or parasitic blood agglutinins, etc.	Isohemagglutinins,Exohemagglutinins,Hemagglutinin
D014764	Viral Proteins	Proteins found in any species of virus.	Gene Products, Viral,Viral Gene Products,Viral Gene Proteins,Viral Protein,Protein, Viral,Proteins, Viral
D015870	Gene Expression	The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.	Expression, Gene,Expressions, Gene,Gene Expressions