-transparent.png){kind=logo}
We examined the possibility that tumor-released products inhibit lymphokine-activated killer cell activation. Lymphokine-activated killer cells from human peripheral blood lymphocytes were activated with recombinant interleukin 2 for 4 days in the presence of malignant effusions or conditioned media from cultured cell lines (10% vol/vol). Eight of 10 malignant effusions/media suppressed the induction of lymphokine-activated killer cell cytotoxicity, as measured in a 4-hour sodium chromate release assay. Seven of 10 effusions/media inhibited lymphokine-activated killer cell proliferation. Suppression was both dose and time dependent. A representative suppressive effusion was fractionated by agarose gel chromatography, treated with detergents disruptive of ionic bonds and lipids, and refractionated using polyacrylamide gel chromatography. Seven suppressive fractions ranging in molecular weight from 1 x 10(5) to 3 x 10(5) d were isolated. It is speculated that this suppressor factor may represent a large multimeric structure with ionic-bonded individual suppressive components.
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
April 1995,
Proceedings of the National Science Council, Republic of China. Part B, Life sciences,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
December 1989,
Japanese journal of cancer research : Gann,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
January 1988,
Immunology today,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
January 1988,
Voprosy onkologii,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
October 1989,
Clinical immunology and immunopathology,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
December 1989,
Journal of immunological methods,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
November 1999,
Nihon rinsho. Japanese journal of clinical medicine,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
February 1991,
Chest,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
June 1987,
Clinical and experimental immunology,
J J Pelton, and
D D Taylor, and
W C Fowler, and
C G Taylor, and
N Z Carp, and
J L Weese
August 1991,
Lancet (London, England),
