Biomaterial Database

Evaluation of aroA deletion mutant of Salmonella enterica subspecies enterica serovar Abortusequi for its vaccine candidate potential. 2009

Javad Alam, and B R Singh, and D Hansda, and V P Singh, and J C Verma

National Salmonella Centre (Vet.), Division of Bacteriology and Mycology Indian Veterinary Research Institute (IVRI), Izatnagar 243122, India.

The present study on a defined deletion aroA mutant (B-26) of Salmonella enterica subspecies enterica serovar Abortusequi (S. Abortusequi) for residual virulence and safety in experimental model revealed that the virulence of the strain was at no difference in any of the cell assays (caprine alveolar macrophages, bovine alveolar macrophages, guinea pig blood mononuclear cells and horse blood mononuclear cells) than that of its parent virulence plasmid cured (S-787) and wild type (E-156) strains. The mutant did not cause any apparent illness in baby guinea pigs (15 days old), adult male and female guinea pigs and also not in pregnant (54-55 days of gestation) guinea pigs through oral (4.2 x 10(9) cfu/ animal) and intramuscular (im) routes (4.2 x 10(7) cfu/ animal). In pregnant females the mutant also induced abortion as its parent (E-156) though to lesser extent (33%) than the parent strain (100%) on inoculation through intravaginal (4.2 x 10(9) cfu/ animal) and intraperitoneal (4.2 x 10(7) cfu/ animal) routes. The babies born from mutant inoculated mothers survived better and were also resistant to intraperitoneal lethal challenge (7.82 x 10(9) cfu/ animal) with 100% protection. Female guinea pigs challenged after 135-165 days of inoculation with the mutant afforded 100% protection from abortion and mortality caused by lethal infection (7.82 x 10(9) cfu/ animal) of wild type S. enterica Abortusequi (E-156). The study revealed that aroA mutant (B-26) was safe through oral and im routes for immunization and afforded 100% protection against salmonellosis for more than 5.5 months in guinea pigs. Although immunization with aroA mutant in experimental model afforded good protection against abortion and mortality induced by S. Abortusequi, further studies are needed in horses to exploit the strain's vaccine potential in the natural host.

UI	MeSH Term	Description	Entries
D009154	Mutation	Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.	Mutations
D011247	Pregnancy	The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.	Gestation,Pregnancies
D002417	Cattle	Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.	Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D005260	Female		Females
D006041	Goats	Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.	Capra,Capras,Goat
D006168	Guinea Pigs	A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.	Cavia,Cavia porcellus,Guinea Pig,Pig, Guinea,Pigs, Guinea
D006736	Horses	Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.	Equus caballus,Equus przewalskii,Horse, Domestic,Domestic Horse,Domestic Horses,Horse,Horses, Domestic
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014436	Typhoid-Paratyphoid Vaccines	Vaccines used to prevent TYPHOID FEVER and/or PARATYPHOID FEVER which are caused by various species of SALMONELLA. Attenuated, subunit, and inactivated forms of the vaccines exist.	Paratyphoid Vaccine,Typhoid Vaccine,Vaccines, Paratyphoid,Vaccines, Typhoid,Paratyphoid Vaccines,Typhoid Paratyphoid Vaccines,Typhoid Vaccines,Vaccine, Paratyphoid,Vaccine, Typhoid,Vaccines, Typhoid-Paratyphoid
D016676	Macrophages, Alveolar	Round, granular, mononuclear phagocytes found in the alveoli of the lungs. They ingest small inhaled particles resulting in degradation and presentation of the antigen to immunocompetent cells.	Alveolar Macrophages,Macrophages, Pulmonary,Pulmonary Macrophages,Macrophage, Pulmonary,Pulmonary Macrophage,Alveolar Macrophage,Macrophage, Alveolar