Biomaterial Database

Motile sperm recovery from fresh and frozen-thawed ejaculates using a swim-up procedure. 1991

J W Graczykowski, and M S Siegel

Department of Obstetrics and Gynecology, University of Southern California-Los Angeles.

The recovery of motile sperm in swim-ups from fresh semen that was washed and centrifuged one time was 33% and after 2 washes and centrifugations was 22%. The recovery rates for frozen-thawed semen were only 7% and 5%, respectively. The straight line velocity of sperm was increased after sperm swim-up, whereas changes to the lateral sperm head movement were not as evident. However, the dramatic loss in motile sperm numbers especially after swim-up from thawed semen indicate that more efficient methods of isolating motile sperm from thawed semen may be required.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D003451	Cryoprotective Agents	Substances that provide protection against the harmful effects of freezing temperatures.	Cryoprotective Agent,Cryoprotective Effect,Cryoprotective Effects,Agent, Cryoprotective,Agents, Cryoprotective,Effect, Cryoprotective,Effects, Cryoprotective
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D012661	Semen	The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.	Seminal Plasma,Plasma, Seminal
D013048	Specimen Handling	Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.	Specimen Collection,Collection, Specimen,Collections, Specimen,Handling, Specimen,Handlings, Specimen,Specimen Collections,Specimen Handlings
D013076	Sperm Count	A count of SPERM in the ejaculum, expressed as number per milliliter.	Sperm Number,Count, Sperm,Counts, Sperm,Number, Sperm,Numbers, Sperm,Sperm Counts,Sperm Numbers
D013081	Sperm Motility	Movement characteristics of SPERMATOZOA in a fresh specimen. It is measured as the percentage of sperms that are moving, and as the percentage of sperms with productive flagellar motion such as rapid, linear, and forward progression.	Motilities, Sperm,Motility, Sperm,Sperm Motilities
D013094	Spermatozoa	Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.	Sperm,Spermatozoon,X-Bearing Sperm,X-Chromosome-Bearing Sperm,Y-Bearing Sperm,Y-Chromosome-Bearing Sperm,Sperm, X-Bearing,Sperm, X-Chromosome-Bearing,Sperm, Y-Bearing,Sperm, Y-Chromosome-Bearing,Sperms, X-Bearing,Sperms, X-Chromosome-Bearing,Sperms, Y-Bearing,Sperms, Y-Chromosome-Bearing,X Bearing Sperm,X Chromosome Bearing Sperm,X-Bearing Sperms,X-Chromosome-Bearing Sperms,Y Bearing Sperm,Y Chromosome Bearing Sperm,Y-Bearing Sperms,Y-Chromosome-Bearing Sperms
D015925	Cryopreservation	Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.	Cryofixation,Cryonic Suspension,Cryonic Suspensions,Suspension, Cryonic