Biomaterial Database

[Study on the interaction of tetrandrine and bovine serum albumin]. 2009

Qiu-Hua Wu, and Shuang-Ju Song, and Chun Wang, and Zhi Wang

College of Science, Agricultural University of Hebei, Baoding 071001, China. wqh69@yahoo.com.cn

The interaction of tetrandrine with bovine serum albumin was studied by fluorescence spectra and ultra-violet spectra. The results showed that tetrandrine could quench the intrinsic fluorescence of BSA. Both static quenching and non-radiation energy transfer were the main reasons for the fluorescence quenching. The quenching constants K(sv) at different temperatures were determined using Stern-Volmer equation. The K(sv) were 1.26 x 10(4) L x mol(-1) (300 K), 1.17 x 10(4) L x mol(-1) (310 K) and 1.12 x 10(4) L x mol(-1) (320 K). According to the Forster theory of non-radiation energy transfer, the binding distances (r) were 3.24 nm (300 K), 3.31 nm (310 K) and 3.50 nm (320 K). The binding constants (KA) between tetrandrine and BSA (300 K: 1.52 x 10(5) L x mol(-1); 310 K: 2.03 x 10(5) L x mol(-1); 320 K: 2.89 x 10(5) L x mol(-1)) and thermodynamic parameters were also obtained. The thermodynamic parameters indicated that the interaction of tetrandrine and BSA was driven mainly by hydrophobic force. Results of synchronous fluorescence spectrum showed that the binding could cause conformational changes of BSA.

UI	MeSH Term	Description	Entries
D011485	Protein Binding	The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.	Plasma Protein Binding Capacity,Binding, Protein
D011487	Protein Conformation	The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).	Conformation, Protein,Conformations, Protein,Protein Conformations
D004735	Energy Transfer	The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.	Transfer, Energy
D012710	Serum Albumin, Bovine	Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)	Fetal Bovine Serum,Fetal Calf Serum,Albumin Bovine,Bovine Albumin,Bovine Serum Albumin,Albumin, Bovine,Albumin, Bovine Serum,Bovine Serum, Fetal,Bovine, Albumin,Calf Serum, Fetal,Serum, Fetal Bovine,Serum, Fetal Calf
D013050	Spectrometry, Fluorescence	Measurement of the intensity and quality of fluorescence.	Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence
D044182	Benzylisoquinolines	ISOQUINOLINES with a benzyl substituent.	Benzyl-Isoquinoline,Benzylisoquinoline,Bis-Benzyl-Isoquinoline,Bis-Benzylisoquinoline,Bisbenzylisoquinoline,Bisbenzylisoquinolines,Benzyl-Isoquinolines,Bis-Benzyl-Isoquinolines,Bis-Benzylisoquinolines,Benzyl Isoquinoline,Benzyl Isoquinolines,Bis Benzyl Isoquinoline,Bis Benzyl Isoquinolines,Bis Benzylisoquinoline,Bis Benzylisoquinolines