BIOMAT Database Logo BIOMAT Database Logo

[Epitope screening of influenza A (H3N2) by using phage display library]. 2009

Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Viral hepatitis research laboratary, Institute of Infectious Disease of P1A 302 Hospital, Beijing 100039, China.

OBJECTIVE To screen the influenza A (H3N2) mimotopes by using phage display library. METHODS Using influenza A (H3N2) monoclonal antibody as selective molecule, a 7 mer phage peptide library was biopanned and positive clones were selected by ELISA, competition assay and DNA sequencing. RESULTS 21 positive clones were chosen for DNA sequencing. From the experiment and sequencing comparison results, one epitope was comfirmed as mimotope of influenza A (H3N2). CONCLUSIONS Influenza A (H3N2) mimotope was obtained by phage peptide library screening. The result provide a new approach for new Influenza virals vaccine development.

UI MeSH Term Description Entries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D053122 Influenza A Virus, H3N2 Subtype A subtype of INFLUENZA A VIRUS comprised of the surface proteins hemagglutinin 3 and neuraminidase 2. The H3N2 subtype was responsible for the Hong Kong flu pandemic of 1968. H3N2 Virus,H3N2v Viruses,Influenza A H3N2, Variant Virus,Influenza Virus, Canine, H3N2 Subtype,H3N2 Viruses,H3N2v Virus,Virus, H3N2,Virus, H3N2v,Viruses, H3N2,Viruses, H3N2v
D018604 Epitope Mapping Methods used for studying the interactions of antibodies with specific regions of protein antigens. Important applications of epitope mapping are found within the area of immunochemistry. Epitope Mappings,Mapping, Epitope,Mappings, Epitope
D019151 Peptide Library A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide. Phage Display Peptide Library,Random Peptide Library,Peptide Phage Display Library,Phage Display Library, Peptide,Synthetic Peptide Combinatorial Library,Synthetic Peptide Library,Libraries, Peptide,Libraries, Random Peptide,Libraries, Synthetic Peptide,Library, Peptide,Library, Random Peptide,Library, Synthetic Peptide,Peptide Libraries,Peptide Libraries, Random,Peptide Libraries, Synthetic,Peptide Library, Random,Peptide Library, Synthetic,Random Peptide Libraries,Synthetic Peptide Libraries

Related Publications

Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Screening E3 substrates using a live phage display library.
January 2013, PloS one,
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Epitope mapping with random phage display library.
January 2014, Methods in molecular biology (Clifton, N.J.),
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
[Study on screening mimicking epitope of ochratoxin A from phage display peptide library].
July 2005, Wei sheng yan jiu = Journal of hygiene research,
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Engineering sortase A by screening a second-generation library using phage display.
July 2017, Journal of peptide science : an official publication of the European Peptide Society,
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Epitope mapping of bovine alpha-lactalbumin using a random phage display peptide library.
May 1997, Biochemical Society transactions,
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Construction and use of a 20-mer phage display epitope library.
January 1998, Methods in molecular biology (Clifton, N.J.),
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Epitope Mapping by Phage Display.
January 2018, Methods in molecular biology (Clifton, N.J.),
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Screening and Identification of a Chicken Dendritic Cell Binding Peptide by Using a Phage Display Library.
January 2019, Frontiers in immunology,
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
Screening of LPS-specific peptides from a phage display library using epoxy beads.
April 2005, Biochemical and biophysical research communications,
Yan-Wei Zhong, and Dong-Ping Xu, and Xiao-Dong Li, and Jiw-Zeng Dai, and Biao Xu, and Le Li
[Screening of short peptides binding to StxB by phage-display library].
October 2006, Wei sheng wu xue bao = Acta microbiologica Sinica,
Copied contents to your clipboard!