Ionic liquids (ILs) are composed entirely of ions and they possess fascinating properties, including low volatility, tunable viscosity and miscibility, and electrolytic conductivity, which make ILs unique and useful for many applications in chemical analysis. The dramatic increase in the number of publications on ILs is indicative of the tremendous interest in this field from analytical chemists. This review summarizes recent efforts in the major subdisciplines of analytical chemistry, including extractions, gas chromatography, liquid chromatography, capillary electrophoresis, mass spectrometry, electrochemistry, sensors, and spectroscopy.