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A series of T-cell proliferations in peripheral blood, bone marrow, or tissue samples were analyzed for clonality. The technique used employs the polymerase chain reaction to amplify portions of the rearranged T-cell receptor beta chain genes, using primers recognizing conserved sequences of the variable, diversity, and joining region segments. We examined 17 cases of T-cell lymphoma or leukemia; a clone was identified in 13 cases (76%) overall and in 7 of 8 cases (87.5%) in which both beta-chain alleles were known to be rearranged, as shown by restriction enzyme analysis. No clonal rearrangements were detected in samples from 13 non-T-cell disorders, including B-cell lymphomas, reactive lymphoid proliferations, and nonlymphoid tumors. This method is useful for detecting clones in thymic and post-thymic T-cell malignancies and has the advantages of being extremely rapid (a result is obtained within hours of the biopsy procedure), requiring no radiolabeling, using only a small amount of tissue, and being applicable to formalin-fixed, paraffin-embedded tissue.
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
November 1990,
Proceedings of the National Academy of Sciences of the United States of America,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
October 2002,
Leukemia,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
January 2003,
Bulletin du cancer,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
June 1974,
The New England journal of medicine,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
June 1995,
Diagnostic molecular pathology : the American journal of surgical pathology, part B,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
August 2023,
Human pathology,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
September 1998,
Journal of immunotherapy (Hagerstown, Md. : 1997),
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
August 2018,
Pathology,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
April 2022,
Histopathology,
K P McCarthy, and
J P Sloane, and
J H Kabarowski, and
E Matutes, and
L M Wiedemann
March 1997,
Human immunology,
