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Diol appended quenchers for fluorescein boronic acid. 2010
Souad A Elfeky, and
Stephen E Flower, and
Naoko Masumoto, and
François D'Hooge, and
Ludivine Labarthe, and
Wenbo Chen, and
Christophe Len, and
Tony D James, and
John S Fossey
School of Chemistry, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Förster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.
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