BIOMAT Database Logo BIOMAT Database Logo

The allergen Bet v 1 in fractions of ambient air deviates from birch pollen counts. 2010

J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
Division of Environmental Dermatology and Allergy, Helmholtz Zentrum München/TUM, ZAUM - Center for Allergy and Environment, Technische Universität München, Munich, Germany. buters@lrz.tum.de

BACKGROUND Proof is lacking that pollen count is representative for allergen exposure, also because allergens were found in nonpollen-bearing fractions of ambient air. OBJECTIVE We monitored simultaneously birch pollen and the major birch pollen allergen Bet v 1 in different size fractions of ambient air from 2004 till 2007 in Munich, Germany. METHODS Air was sampled with a ChemVol high-volume cascade impactor equipped with stages for particulate matter (PM)>10 microm, 10 microm>PM>2.5 microm, and 2.5 microm>PM>0.12 microm. Allergen was determined with a Bet v 1-specific ELISA. Pollen count was assessed with a Burkard pollen trap. We also measured the development of allergen in pollen during ripening. RESULTS About 93 +/- 3% of Bet v 1 was found in the PM > 10 microm fraction, the fraction containing birch pollen. We did not measure any Bet v 1 in 2.5 microm > PM > 0.12 microm. Either in Munich no allergen was in this fraction or the allergen was absorbed to diesel soot particles that also deposit in this fraction. Pollen released 115% more Bet v 1 in 2007 than in 2004. Also within 1 year, the release of allergen from the same amount of pollen varied more than 10-fold between different days. This difference was explained by a rapidly increasing expression of Bet v 1 in pollen in the week just before pollination. Depending on the day the pollen is released during ripening, its potency varies. CONCLUSIONS In general, pollen count and allergen in ambient air follow the same temporal trends. However, because a 10-fold difference can exist in allergen potency of birch pollen, symptoms might be difficult to correlate with pollen counts, but perhaps better with allergen exposure.

UI MeSH Term Description Entries
D011058 Pollen The fertilizing element of plants that contains the male GAMETOPHYTES. Male Gametes, Plant,Male Gametophytes,Microspores, Plant,Plant Microspores,Pollen Grains,Gamete, Plant Male,Gametes, Plant Male,Gametophyte, Male,Gametophytes, Male,Grain, Pollen,Grains, Pollen,Male Gamete, Plant,Male Gametophyte,Microspore, Plant,Plant Male Gamete,Plant Male Gametes,Plant Microspore,Pollen Grain
D004784 Environmental Monitoring The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment. Monitoring, Environmental,Environmental Surveillance,Surveillance, Environmental
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005858 Germany A country in central Europe, bordering the Baltic Sea and the North Sea, between the Netherlands and Poland, south of Denmark. The capital is Berlin.
D006255 Rhinitis, Allergic, Seasonal Allergic rhinitis that occurs at the same time every year. It is characterized by acute CONJUNCTIVITIS with lacrimation and ITCHING, and regarded as an allergic condition triggered by specific ALLERGENS. Hay Fever,Pollen Allergy,Pollinosis,Seasonal Allergic Rhinitis,Hayfever,Allergic Rhinitides, Seasonal,Allergic Rhinitis, Seasonal,Allergies, Pollen,Allergy, Pollen,Fever, Hay,Pollen Allergies,Pollinoses,Rhinitides, Seasonal Allergic,Rhinitis, Seasonal Allergic,Seasonal Allergic Rhinitides
D000388 Air The mixture of gases present in the earth's atmosphere consisting of oxygen, nitrogen, carbon dioxide, and small amounts of other gases.
D052179 Antigens, Plant Substances found in PLANTS that have antigenic activity. Plant Antigen,Plant Antigens,Antigen, Plant
D029662 Betula A plant genus of the family BETULACEAE. The tree has smooth, resinous, varicolored or white bark, marked by horizontal pores (lenticels), which usually peels horizontally in thin sheets. Birch,Betulas,Birchs

Related Publications

J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
The major birch pollen allergen, Bet v 1, shows ribonuclease activity.
January 1996, Planta,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
Characterisation of recombinant isoforms of birch pollen allergen Bet v 1.
January 1996, Advances in experimental medicine and biology,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
Determination of nitration degrees for the birch pollen allergen Bet v 1.
November 2013, Analytical and bioanalytical chemistry,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
The major allergen from birch tree pollen, Bet v 1, binds and permeabilizes membranes.
March 2007, Biochemistry,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
The History and Science of the Major Birch Pollen Allergen Bet v 1.
July 2023, Biomolecules,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
Dynamics Rationalize Proteolytic Susceptibility of the Major Birch Pollen Allergen Bet v 1.
January 2020, Frontiers in molecular biosciences,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
Functional analysis of birch pollen allergen Bet v 1-specific regulatory T cells.
January 2007, Journal of immunology (Baltimore, Md. : 1950),
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
Ligand Recognition of the Major Birch Pollen Allergen Bet v 1 is Isoform Dependent.
January 2015, PloS one,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
Stabilization of the dimeric birch pollen allergen Bet v 1 impacts its immunological properties.
January 2014, The Journal of biological chemistry,
J T M Buters, and I Weichenmeier, and S Ochs, and G Pusch, and W Kreyling, and A J F Boere, and W Schober, and H Behrendt
High-level expression and purification of the major birch pollen allergen, Bet v 1.
February 1997, Protein expression and purification,
Copied contents to your clipboard!