Biomaterial Database

Purification and characterization of a plant peroxisomal delta 2, delta 3-enoyl-CoA isomerase acting on 3-cis-enoyl-CoA and 3-trans-enoyl-CoA. 1991

K Engeland, and H Kindl

Department of Biochemistry, Philipps-University, Marburg, Federal Republic of Germany.

A delta 3, delta 2-enoyl-CoA isomerase was extracted from fat-degrading cotyledons of cucumber seedlings. The enzyme is required for the degradation of cis-unsaturated fatty acids, e.g. linoleic acid being present in the storage tissue of cucumber seedlings in high amounts. The delta 3, delta 2-enoyl-CoA isomerase was exclusively localized within peroxisomes. Its purification included chromatography on a hydrophobic matrix, a cation exchanger, and on hydroxylapatite. 17,000-fold purification yielded a protein of apparent homogeneity. The isomerase is a homodimer with a Mr of 50,000 and an isoelectric point of pH approximately 8.1. Delta 3, delta 2-Enoyl-CoA isomerase reversibly catalyzes the conversion of both cis-3-enoyl-CoA and trans-3-enoyl-CoA into trans-2-enoyl-CoA. The isomerase exhibited optimal activity at pH 9 and was active with 3-enoyl-CoA species having chain lengths of C6-C12, with cis-hexanoyl-CoA being the most effective substrate. The purified enzyme did not show enoyl-CoA hydratase activity.

UI	MeSH Term	Description	Entries
D007535	Isomerases	A class of enzymes that catalyze geometric or structural changes within a molecule to form a single product. The reactions do not involve a net change in the concentrations of compounds other than the substrate and the product.(from Dorland, 28th ed) EC 5.	Isomerase
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D008830	Microbodies	Electron-dense cytoplasmic particles bounded by a single membrane, such as PEROXISOMES; GLYOXYSOMES; and glycosomes.	Glycosomes,Glycosome,Microbody
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D005227	Fatty Acids	Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)	Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D019748	Carbon-Carbon Double Bond Isomerases	Enzymes that catalyze the shifting of a carbon-carbon double bond from one position to another within the same molecule. EC 5.3.3.	C-C Double Bond Isomerases,Carbon Carbon Double Bond Isomerases
D064006	Dodecenoyl-CoA Isomerase	A carbon-carbon double bond isomerase that catalyzes the movement double bond from C3 to C2 of an unsaturated acyl-CoA. The enzyme plays a key role in allowing acyl-CoA substrates to re-enter the beta-oxidation pathway.	3,2-trans-Enoyl-CoA Isomerase,Acetylene-Allene Isomerase,Dodecenoyl-CoA delta-Isomerase,Dodecenoyl-Coenzyme A delta-Isomerase,Enoyl-CoA Isomerase,delta(3), delta(2)-Enoyl-CoA Isomerase,3,2 trans Enoyl CoA Isomerase,Acetylene Allene Isomerase,Dodecenoyl CoA Isomerase,Dodecenoyl CoA delta Isomerase,Dodecenoyl Coenzyme A delta Isomerase,Enoyl CoA Isomerase,Isomerase, 3,2-trans-Enoyl-CoA,Isomerase, Acetylene-Allene,Isomerase, Dodecenoyl-CoA,Isomerase, Enoyl-CoA,delta-Isomerase, Dodecenoyl-CoA,delta-Isomerase, Dodecenoyl-Coenzyme A