OBJECTIVE To analyze the effect of LASIK on visual quality of anisometropia, and evaluate its clinical value in the view of visual quality. METHODS Prospective observational case series. Assayed the naked vision, glasses-corrected vision and binocular vision of 45 cases with anisometropia >or= 2.25D before and after the operation of LASIK. RESULTS 91.57% of the eyes after the operation reached the vision >or= 0.8, which says a significant improvement for binocular vision after the operation (P < 0.05). There was a significant difference on diopter between the pre-operation and post-operation (P < 0.05). As for anisometropia, there was no significant difference between simultaneous binocular visions (P = 0.431), but there was of great significance among combined, short and long distance stereopsis visions (P = 0.000). Binocular vision deteriorated as anisometropia increased (P < 0.05). The short distance stereopsis visions of LASIK-treated myopic anisometropia were better than that of glasses-corrected patients (P < 0.05). CONCLUSIONS The operation of LASIK can improve the visual quality and resume the binocular vision. LASIK can correct anisometropia and its therapeutic efficacy deserves to confirm.