Development of a noncompetitive phage anti-immunocomplex assay for brominated diphenyl ether 47. 2010

Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
Department of Entomology and UCD Cancer Center, University of California, Davis, CA 95616, USA.

We present a new application of the noncompetitive phage anti-immunocomplex assay (PHAIA) by converting an existing competitive assay to a versatile noncompetitive sandwich-type format using immunocomplex binding phage-borne peptides to detect the brominated flame retardant, brominated diphenyl ether 47 (BDE 47). Three phage-displayed 9-mer disulfide-constrained peptides that recognize the BDE 47-polyclonal antibody immunocomplex were isolated. The resulting PHAIAs showed variable sensitivities, and the most sensitive peptide had a dose-response curve with an SC(50) (concentration of analyte producing 50% saturation of the signal) of 0.7ng/ml BDE 47 and a linear range of 0.3-2ng/ml, which was nearly identical to the best heterologous competitive format (IC(50) of 1.8ng/ml, linear range of 0.4-8.5/ml). However, the PHAIA was 1400-fold better than homologous competitive assay. The validation of the PHAIA with extracts of house furniture foam as well as human and calf sera spiked with BDE 47 showed overall recovery of 80-113%. The PHAIA was adapted to a dipstick format (limit of detection of 3.0ng/ml), and a blind test with six random extracts of local house furniture foams showed that the results of the PHAIA and dipstick assay were consistent, giving the same positive and negative detection.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000906 Antibodies Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
D012997 Solvents Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed) Solvent
D055768 Halogenated Diphenyl Ethers Compounds that contain two halogenated benzene rings linked via an OXYGEN atom. Many polybrominated diphenyl ethers are used as FLAME RETARDANTS. Brominated Diphenyl Ethers,Chlorinated Diphenyl Ethers,Fluorinated Diphenyl Ethers,Iodinated Diphenyl Ethers,PBDE Compounds,PBDEs,PCDE Compounds,PCDEs,Polybrominated Diphenyl Ethers,Diphenyl Ethers, Brominated,Diphenyl Ethers, Chlorinated,Diphenyl Ethers, Fluorinated,Diphenyl Ethers, Halogenated,Diphenyl Ethers, Iodinated,Diphenyl Ethers, Polybrominated,Ethers, Brominated Diphenyl,Ethers, Chlorinated Diphenyl,Ethers, Fluorinated Diphenyl,Ethers, Halogenated Diphenyl,Ethers, Iodinated Diphenyl,Ethers, Polybrominated Diphenyl
D019151 Peptide Library A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide. Phage Display Peptide Library,Random Peptide Library,Peptide Phage Display Library,Phage Display Library, Peptide,Synthetic Peptide Combinatorial Library,Synthetic Peptide Library,Libraries, Peptide,Libraries, Random Peptide,Libraries, Synthetic Peptide,Library, Peptide,Library, Random Peptide,Library, Synthetic Peptide,Peptide Libraries,Peptide Libraries, Random,Peptide Libraries, Synthetic,Peptide Library, Random,Peptide Library, Synthetic,Random Peptide Libraries,Synthetic Peptide Libraries

Related Publications

Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
June 2022, Analytical biochemistry,
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
February 2015, Biosensors & bioelectronics,
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
October 2014, Analytical chemistry,
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
January 2011, Analytical chemistry,
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
January 2001, Environmental toxicology and chemistry,
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
April 2020, Reproductive toxicology (Elmsford, N.Y.),
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
October 2007, Analytical chemistry,
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
November 1996, Journal of natural products,
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
September 2016, Aquatic toxicology (Amsterdam, Netherlands),
Hee-Joo Kim, and Martin A Rossotti, and Ki Chang Ahn, and Gualberto G González-Sapienza, and Shirley J Gee, and Ruthie Musker, and Bruce D Hammock
March 2015, Chemical research in toxicology,
Copied contents to your clipboard!