BIOMAT Database Logo BIOMAT Database Logo

Different thymosin Beta 4 immunoreactivity in foetal and adult gastrointestinal tract. 2010

Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Divisione di Anatomia Patologica, Dipartimento di Citomorfologia, University of Cagliari, Cagliari, Italy.

BACKGROUND Thymosin beta 4 (Tbeta(4)) is a member of beta-thymosins, a family of peptides that play essential roles in many cellular functions. A recent study from our group suggested a role for Tbeta(4) in the development of human salivary glands. The aim of this study was to analyze the expression of Tbeta(4) in the human gut during development, and in the adult. RESULTS Immunolocalization of Tbeta(4) was studied in autoptic samples of tongue, oesophagus, stomach, ileum, colon, liver and pancreas obtained from two human foetuses and two adults. Tbeta(4) appeared unevenly distributed, with marked differences between foetuses and adults. In the stomach, superficial epithelium was positive in foetuses and negative in adults. Ileal enterocytes were strongly positive in the adult and weakly positive in the foetuses. An increase in reactivity for Tbeta(4) was observed in superficial colon epithelium of adults as compared with the foetuses. Striking differences were found between foetal and adult liver: the former showed a very low reactivity for Tbeta(4) while in the adult we observed a strong reactivity in the vast majority of the hepatocytes. A peculiar pattern was found in the pancreas, with the strongest reactivity observed in foetal and adult islet cells. CONCLUSIONS Our data show a strong expression of Tbeta(4) in the human gut and in endocrine pancreas during development. The observed differential expression of Tbeta(4) suggests specific roles of the peptide in the gut of foetuses and adults. The observed heterogeneity of Tbeta(4) expression in the foetal life, ranging from a very rare detection in liver cells up to a diffuse reactivity in endocrine pancreas, should be taken into account when the role of Tbeta(4) in the development of human embryo is assessed. Future studies are needed to shed light on the link between Tbeta(4) and organogenesis.

UI MeSH Term Description Entries
D007150 Immunohistochemistry Histochemical localization of immunoreactive substances using labeled antibodies as reagents. Immunocytochemistry,Immunogold Techniques,Immunogold-Silver Techniques,Immunohistocytochemistry,Immunolabeling Techniques,Immunogold Technics,Immunogold-Silver Technics,Immunolabeling Technics,Immunogold Silver Technics,Immunogold Silver Techniques,Immunogold Technic,Immunogold Technique,Immunogold-Silver Technic,Immunogold-Silver Technique,Immunolabeling Technic,Immunolabeling Technique,Technic, Immunogold,Technic, Immunogold-Silver,Technic, Immunolabeling,Technics, Immunogold,Technics, Immunogold-Silver,Technics, Immunolabeling,Technique, Immunogold,Technique, Immunogold-Silver,Technique, Immunolabeling,Techniques, Immunogold,Techniques, Immunogold-Silver,Techniques, Immunolabeling
D007515 Islets of Langerhans Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN. Islands of Langerhans,Islet Cells,Nesidioblasts,Pancreas, Endocrine,Pancreatic Islets,Cell, Islet,Cells, Islet,Endocrine Pancreas,Islet Cell,Islet, Pancreatic,Islets, Pancreatic,Langerhans Islands,Langerhans Islets,Nesidioblast,Pancreatic Islet
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D004848 Epithelium The layers of EPITHELIAL CELLS which cover the inner and outer surfaces of the cutaneous, mucus, and serous tissues and glands of the body. Mesothelium,Epithelial Tissue,Mesothelial Tissue,Epithelial Tissues,Mesothelial Tissues,Tissue, Epithelial,Tissue, Mesothelial,Tissues, Epithelial,Tissues, Mesothelial
D005260 Female Females
D005753 Gastric Mucosa Lining of the STOMACH, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. The surface cells produce MUCUS that protects the stomach from attack by digestive acid and enzymes. When the epithelium invaginates into the LAMINA PROPRIA at various region of the stomach (CARDIA; GASTRIC FUNDUS; and PYLORUS), different tubular gastric glands are formed. These glands consist of cells that secrete mucus, enzymes, HYDROCHLORIC ACID, or hormones. Cardiac Glands,Gastric Glands,Pyloric Glands,Cardiac Gland,Gastric Gland,Gastric Mucosas,Gland, Cardiac,Gland, Gastric,Gland, Pyloric,Glands, Cardiac,Glands, Gastric,Glands, Pyloric,Mucosa, Gastric,Mucosas, Gastric,Pyloric Gland
D005865 Gestational Age The age of the conceptus, beginning from the time of FERTILIZATION. In clinical obstetrics, the gestational age is often estimated from the onset of the last MENSTRUATION which is about 2 weeks before OVULATION and fertilization. It is also estimated to begin from fertilization, estrus, coitus, or artificial insemination. Embryologic Age,Fetal Maturity, Chronologic,Chronologic Fetal Maturity,Fetal Age,Maturity, Chronologic Fetal,Age, Embryologic,Age, Fetal,Age, Gestational,Ages, Embryologic,Ages, Fetal,Ages, Gestational,Embryologic Ages,Fetal Ages,Gestational Ages
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly

Related Publications

Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Thymosin beta 4.
January 1985, Methods in enzymology,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Thymosin beta 4 interactions.
January 2003, Vitamins and hormones,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Thymosin beta 4 (T beta 4) in activated platelets.
August 1993, European journal of cell biology,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Interaction of G-actin with thymosin beta 4 and its variants thymosin beta 9 and thymosin beta met9.
June 1994, Journal of muscle research and cell motility,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Actin-sequestering ability of thymosin beta 4, thymosin beta 4 fragments, and thymosin beta 4-like peptides as assessed by the DNase I inhibition assay.
February 1993, Biological chemistry Hoppe-Seyler,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Cardioprotection by Thymosin Beta 4.
January 2016, Vitamins and hormones,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Thymosin beta 4-like peptides.
January 1985, Methods in enzymology,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Synthesis of thymosin beta 4.
October 1981, International journal of peptide and protein research,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Thymosin beta 10 and thymosin beta 4 are both actin monomer sequestering proteins.
January 1993, The Journal of biological chemistry,
Sonia Nemolato, and Tiziana Cabras, and Flaviana Cau, and Mattia Umberto Fanari, and Daniela Fanni, and Barbara Manconi, and Irene Messana, and Massimo Castagnola, and Gavino Faa
Immunoreactivity of transforming growth factor alpha in the normal adult gastrointestinal tract.
May 1992, Gut,
Copied contents to your clipboard!