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Six major acidic glycosphingolipids were isolated from human amnion using DEAE Sephadex A-25 and silica beads column chromatography. The structures of these glycosphingolipids were determined by methylation analysis, TLC immunostaining and/or negative ion FAB-MS, and were concluded to be II3 alpha NeuAcLacCer(GM3), IV3 alpha NeuAcnLc4-Cer (sialyl[alpha 2-3]paragloboside), IV6 alpha NeuAcnLc4Cer (sialyl[alpha 2-6]paragloboside), IV3 alpha NeuAcIII4 alpha FucLc4Cer (sialyl Lea), VI3 alpha NeuAcnLc6Cer (i-ganglioside) and II3 alpha (NeuAc alpha 2----8NeuAc)LacCer (GD3). In addition, several minor glycosphingolipids were detected with specific monoclonal antibodies, including glycolipids with NeuAc alpha 2-3Gal beta 1-4GlcNAc-beta 1- or NeuAc alpha 2-6Gal beta 1-4GlcNAc beta 1- determinant. Our results show that the glycosphingolipids of human amnion are characterized by having mainly type II chain analogues and onco-fetal antigens.
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
October 1990,
The Japanese journal of experimental medicine,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
September 2019,
Analytical biochemistry,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
March 1994,
European journal of obstetrics, gynecology, and reproductive biology,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
March 1995,
Glycobiology,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
June 2000,
European journal of biochemistry,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
February 1983,
Journal of lipid research,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
May 1985,
Archives of biochemistry and biophysics,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
October 1986,
Bioorganicheskaia khimiia,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
December 1990,
Cancer research,
K Asano, and
M Suzuki, and
S Handa, and
M Oshima
October 1974,
Lipids,
