Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins. 2010

F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
Departamento de Ciencia y Tecnología de los Alimentos, Universidad de Santiago de Chile (USACH), Santiago, Chile.

OBJECTIVE To study genomic and phenotypic changes in wine yeasts produced in short time periods analysing yeast strains possibly derived from commercial strains recently dispersed. RESULTS We conducted a genomic and phenotypic comparison between the commercial yeast strain EC1118 and two novel strains (LV CB and L-957) isolated from different wine areas industrially intervened <20 years ago. Molecular analysis by amplified fragment length polymorphism (AFLP) and RAPD-PCR was not able to distinguish between these strains. However, comparative genomic hybridization (aCGH) showed discrete DNA gains and losses that allowed unequivocal identification of the strains. Furthermore, analysis of aCGH data supports the hypothesis that strains LV CB and L-957 are derivatives from strain EC1118. Finally, scarce phenotypic differences in physiological and metabolic parameters were found among the strains. CONCLUSIONS The wine yeasts have a very dynamic genome that accumulates changes in short time periods. These changes permit the unique genomic identification of the strains. CONCLUSIONS This study permits the evaluation of microevolutive events in wine yeasts and its relationship with the phenotype in this species.

UI MeSH Term Description Entries
D010641 Phenotype The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment. Phenotypes
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D014920 Wine Fermented juice of fresh grapes or of other fruit or plant products used as a beverage. Wines
D016681 Genome, Fungal The complete gene complement contained in a set of chromosomes in a fungus. Fungal Genome,Fungal Genomes,Genomes, Fungal
D054458 Amplified Fragment Length Polymorphism Analysis The detection of RESTRICTION FRAGMENT LENGTH POLYMORPHISMS by selective PCR amplification of restriction fragments derived from genomic DNA followed by electrophoretic analysis of the amplified restriction fragments. AFLP Analysis,AFLP Analyses,Analyses, AFLP,Analysis, AFLP
D055028 Comparative Genomic Hybridization A method for comparing two sets of chromosomal DNA by analyzing differences in the copy number and location of specific sequences. It is used to look for large sequence changes such as deletions, duplications, amplifications, or translocations. Array Comparative Genomic Hybridization,Array-Based Comparative Genomic Hybridization,Comparative Genome Hybridization,Array Based Comparative Genomic Hybridization,Comparative Genome Hybridizations,Comparative Genomic Hybridizations,Genome Hybridization, Comparative,Genome Hybridizations, Comparative,Genomic Hybridization, Comparative,Genomic Hybridizations, Comparative,Hybridization, Comparative Genome,Hybridization, Comparative Genomic,Hybridizations, Comparative Genome,Hybridizations, Comparative Genomic
D018628 Gene Dosage The number of copies of a given gene present in the cell of an organism. An increase in gene dosage (by GENE DUPLICATION for example) can result in higher levels of gene product formation. GENE DOSAGE COMPENSATION mechanisms result in adjustments to the level GENE EXPRESSION when there are changes or differences in gene dosage. Gene Copy Number,Copy Number, Gene,Copy Numbers, Gene,Dosage, Gene,Dosages, Gene,Gene Copy Numbers,Gene Dosages,Number, Gene Copy,Numbers, Gene Copy
D019105 Random Amplified Polymorphic DNA Technique Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes. RAPD Technique,RAPD-PCR,Arbitrarily Primed PCR Reaction,RAPD Technic,Random Amplified Polymorphic DNA Technic,RAPD Technics,RAPD Techniques
D029701 Saccharomyces cerevisiae Proteins Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes. Baker's Yeast Proteins,S cerevisiae Proteins

Related Publications

F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
December 2016, Biochemistry. Biokhimiia,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
August 2012, BMC genomics,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
January 2008, Applied microbiology and biotechnology,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
February 2018, FEMS yeast research,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
January 2016, Applied and environmental microbiology,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
January 2004, Journal of applied microbiology,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
April 2006, FEMS microbiology letters,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
January 2013, PloS one,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
April 2010, International journal of food microbiology,
F Salinas, and D Mandaković, and U Urzua, and A Massera, and S Miras, and M Combina, and M Angelica Ganga, and C Martínez
April 1999, Antonie van Leeuwenhoek,
Copied contents to your clipboard!