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In our study we confirmed the potential of human umbilical cord blood cells to differentiate into insulin-producing cells following transplantation into immunocompromised mice. The average number of C-peptide-positive human cells per animal was 18 +/- 13 as assessed by immunofluorescence staining and fluorescence in situ hybridization specific for human ALU sequence. Differentiation into insulin-producing cells was further confirmed by reverse transcription-polymerase chain reaction specific for human insulin mRNA. Successful differentiation required sublethal irradiation of xenogeneic recipient at least at a dose of 3 Gy. However, transplantation of human umbilical cord blood cells did not improve hyperglycaemia in diabetic animals. The results of our study show that human umbilical cord blood may be considered as a potential source of stem cells for treatment of diabetes mellitus.
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
October 2005,
Stem cells (Dayton, Ohio),
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
March 2010,
Chinese medical journal,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
June 2014,
Differentiation; research in biological diversity,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
October 2008,
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
December 2011,
International journal of molecular medicine,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
February 2007,
Journal of cellular biochemistry,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
January 2012,
Cell transplantation,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
May 2011,
Chinese medical journal,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
October 2015,
Biochemical and biophysical research communications,
T Koblas, and
K Zacharovová, and
Z Berková, and
I Leontovic, and
E Dovolilová, and
L Zámecník, and
F Saudek
December 2008,
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi,
