BIOMAT Database Logo BIOMAT Database Logo

RNF220, an E3 ubiquitin ligase that targets Sin3B for ubiquitination. 2010

Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
CAS-Max Planck Junior Scientist Group on Developmental Biology, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, 32 Jiaochang East Road, Kunming 650223, China.

Modification of proteins by ubiquitination plays important roles in various cellular processes. During this process, the target specificity is determined by ubiquitin ligases. Here we identify RNF220 (RING finger protein 220) as a novel ubiquitin ligase for Sin3B. As a conserved RING protein, RNF220 can bind E2 and mediate auto-ubiquitination of itself. Through a yeast two-hybrid screen, we isolated Sin3B as one of its targets, which is a scaffold protein of the Sin3/HDAC (histone deacetylase) corepressor complex. RNF220 specifically interacts with Sin3B both in vitro and in vivo. Sin3B can be regulated by the ubiquitin-proteasome system. Co-expression of RNF220 promotes the ubiquitination and proteasomal degradation of Sin3B. Taken together, these results reveal a new mechanism for regulating the Sin3/HDAC complex.

UI MeSH Term Description Entries
D012097 Repressor Proteins Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release. Repressor Molecules,Transcriptional Silencing Factors,Proteins, Repressor,Silencing Factors, Transcriptional
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D044767 Ubiquitin-Protein Ligases A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes. Ubiquitin-Protein Ligase,E3 Ligase,E3 Ubiquitin Ligase,Ubiquitin Ligase E3,Ubiquitin-Protein Ligase E3,Ligase E3, Ubiquitin,Ligase E3, Ubiquitin-Protein,Ligase, E3,Ligase, E3 Ubiquitin,Ligase, Ubiquitin-Protein,Ligases, Ubiquitin-Protein,Ubiquitin Ligase, E3,Ubiquitin Protein Ligase,Ubiquitin Protein Ligase E3,Ubiquitin Protein Ligases
D051379 Mice The common name for the genus Mus. Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus
D054875 Ubiquitination The act of ligating UBIQUITINS to PROTEINS to form ubiquitin-protein ligase complexes to label proteins for transport to the PROTEASOME ENDOPEPTIDASE COMPLEX where proteolysis occurs. Ubiquitylation
D020798 Two-Hybrid System Techniques Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions. One-Hybrid System Techniques,Reverse One-Hybrid System Techniques,Reverse Two-Hybrid System Techniques,Three-Hybrid System Techniques,Yeast Two-Hybrid Assay,Yeast Two-Hybrid System Techniques,One-Hybrid System Technics,Reverse Three-Hybrid System Techniques,Three-Hybrid System Technics,Tri-Hybrid System Techniques,Two-Hybrid Assay,Two-Hybrid Method,Two-Hybrid System Technics,Yeast One-Hybrid System Techniques,Yeast Three-Hybrid Assay,Yeast Three-Hybrid System,Yeast Three-Hybrid System Techniques,Yeast Two-Hybrid System,n-Hybrid System Techniques,Assay, Two-Hybrid,Assay, Yeast Three-Hybrid,Assay, Yeast Two-Hybrid,Assays, Two-Hybrid,Assays, Yeast Three-Hybrid,Assays, Yeast Two-Hybrid,Method, Two-Hybrid,Methods, Two-Hybrid,One Hybrid System Technics,One Hybrid System Techniques,One-Hybrid System Technic,One-Hybrid System Technique,Reverse One Hybrid System Techniques,Reverse Three Hybrid System Techniques,Reverse Two Hybrid System Techniques,System Technique, n-Hybrid,System Techniques, n-Hybrid,System, Yeast Three-Hybrid,System, Yeast Two-Hybrid,Systems, Yeast Three-Hybrid,Systems, Yeast Two-Hybrid,Technic, One-Hybrid System,Technic, Three-Hybrid System,Technic, Two-Hybrid System,Technics, One-Hybrid System,Technics, Three-Hybrid System,Technics, Two-Hybrid System,Technique, One-Hybrid System,Technique, Three-Hybrid System,Technique, Tri-Hybrid System,Technique, Two-Hybrid System,Technique, n-Hybrid System,Techniques, One-Hybrid System,Techniques, Three-Hybrid System,Techniques, Tri-Hybrid System,Techniques, Two-Hybrid System,Techniques, n-Hybrid System,Three Hybrid System Technics,Three Hybrid System Techniques,Three-Hybrid Assay, Yeast,Three-Hybrid Assays, Yeast,Three-Hybrid System Technic,Three-Hybrid System Technique,Three-Hybrid System, Yeast,Three-Hybrid Systems, Yeast,Tri Hybrid System Techniques,Tri-Hybrid System Technique,Two Hybrid Assay,Two Hybrid Method,Two Hybrid System Technics,Two Hybrid System Techniques,Two-Hybrid Assay, Yeast,Two-Hybrid Assays,Two-Hybrid Assays, Yeast,Two-Hybrid Methods,Two-Hybrid System Technic,Two-Hybrid System Technique,Two-Hybrid System, Yeast,Two-Hybrid Systems, Yeast,Yeast One Hybrid System Techniques,Yeast Three Hybrid Assay,Yeast Three Hybrid System,Yeast Three Hybrid System Techniques,Yeast Three-Hybrid Assays,Yeast Three-Hybrid Systems,Yeast Two Hybrid Assay,Yeast Two Hybrid System,Yeast Two Hybrid System Techniques,Yeast Two-Hybrid Assays,Yeast Two-Hybrid Systems,n Hybrid System Techniques,n-Hybrid System Technique
D041681 NIH 3T3 Cells A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/) 3T3 Cells, NIH,NIH-3T3 Cells,3T3 Cell, NIH,Cell, NIH 3T3,Cell, NIH-3T3,Cells, NIH 3T3,Cells, NIH-3T3,NIH 3T3 Cell,NIH-3T3 Cell

Related Publications

Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
Wwp2, an E3 ubiquitin ligase that targets transcription factor Oct-4 for ubiquitination.
May 2004, The Journal of biological chemistry,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission.
September 2022, Science advances,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
The E3 ubiquitin ligase RNF5 targets virus-induced signaling adaptor for ubiquitination and degradation.
June 2010, Journal of immunology (Baltimore, Md. : 1950),
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
SCFβ-TRCP E3 ubiquitin ligase targets the tumor suppressor ZNRF3 for ubiquitination and degradation.
October 2018, Protein & cell,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
Psh1 is an E3 ubiquitin ligase that targets the centromeric histone variant Cse4.
November 2010, Molecular cell,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
Staring, a novel E3 ubiquitin-protein ligase that targets syntaxin 1 for degradation.
September 2002, The Journal of biological chemistry,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
E3 ubiquitin ligase Siah1 aggravates NAFLD through Scp2 ubiquitination.
November 2023, International immunopharmacology,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation.
July 2019, Scientific reports,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
The many faces of the E3 ubiquitin ligase, RNF220, in neural development and beyond.
February 2022, Development, growth & differentiation,
Qinghua Kong, and Wanli Zeng, and Jingyang Wu, and Wanling Hu, and Chaocui Li, and Bingyu Mao
HERC2 is an E3 ligase that targets BRCA1 for degradation.
August 2010, Cancer research,
Copied contents to your clipboard!