Determination of potassium, sodium, calcium and magnesium in total parenteral nutrition formulations by capillary electrophoresis with contactless conductivity detection. 2010

Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
Hôpitaux Universitaire de Genève-Pharmacie, Rue Gabrielle-Perret-Gentil 4, 1211 Genève 14, Switzerland.

A simple method based on capillary electrophoresis with a capacitively coupled contactless conductivity detector (CE-C(4)D) was developed for the determination of potassium, sodium, calcium and magnesium in parenteral nutrition formulations. A hydro-organic mixture, consisting of 100 mM Tris-acetate buffer at pH 4.5 and acetonitrile (80:20, v/v), was selected as the background electrolyte. The applied voltage was 30 kV, and sample injection was performed in hydrodynamic mode. All analyses were carried out in a fused silica capillary with an internal diameter of 50 microm and a total length of 64.5 cm. Under these conditions, complete separation between all cations was achieved in less than 4 min. The CE-C(4)D method was validated, and trueness values between 98.6% and 101.8% were obtained with repeatability and intermediate precision values of 0.4-1.3% and 0.8-1.8%, respectively. Therefore, this method was found to be appropriate for controlling potassium, sodium, calcium and magnesium in parenteral nutrition formulations and successfully applied in daily quality control at the Geneva University Hospitals.

UI MeSH Term Description Entries
D008274 Magnesium A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
D010289 Parenteral Nutrition, Total The delivery of nutrients for assimilation and utilization by a patient whose sole source of nutrients is via solutions administered intravenously, subcutaneously, or by some other non-alimentary route. The basic components of TPN solutions are protein hydrolysates or free amino acid mixtures, monosaccharides, and electrolytes. Components are selected for their ability to reverse catabolism, promote anabolism, and build structural proteins. Hyperalimentation, Parenteral,Intravenous Hyperalimentation,Nutrition, Total Parenteral,Parenteral Hyperalimentation,Total Parenteral Nutrition,Hyperalimentation, Intravenous
D011188 Potassium An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
D011786 Quality Control A system for verifying and maintaining a desired level of quality in a product or process by careful planning, use of proper equipment, continued inspection, and corrective action as required. (Random House Unabridged Dictionary, 2d ed) Control, Quality,Controls, Quality,Quality Controls
D002118 Calcium A basic element found in nearly all tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes. Coagulation Factor IV,Factor IV,Blood Coagulation Factor IV,Calcium-40,Calcium 40,Factor IV, Coagulation
D002412 Cations Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis. Cation
D012964 Sodium A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23. Sodium Ion Level,Sodium-23,Ion Level, Sodium,Level, Sodium Ion,Sodium 23
D019075 Electrophoresis, Capillary A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992) Capillary Zone Electrophoresis,Capillary Electrophoreses,Capillary Electrophoresis,Capillary Zone Electrophoreses,Electrophoreses, Capillary,Electrophoreses, Capillary Zone,Electrophoresis, Capillary Zone,Zone Electrophoreses, Capillary,Zone Electrophoresis, Capillary
D019999 Pharmaceutical Solutions Homogeneous liquid preparations that contain one or more chemical substances dissolved, i.e., molecularly dispersed, in a suitable solvent or mixture of mutually miscible solvents. For reasons of their ingredients, method of preparation, or use, they do not fall into another group of products. Pharmaceutic Solutions,Solutions, Pharmaceutical,Solutions, Pharmaceutic

Related Publications

Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
January 2013, Chimia,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
June 2003, Electrophoresis,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
January 2010, Electrophoresis,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
January 2008, Methods in molecular biology (Clifton, N.J.),
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
October 2012, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
February 2011, Se pu = Chinese journal of chromatography,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
May 2006, Electrophoresis,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
March 2015, Journal of separation science,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
February 1998, Analytical chemistry,
Susanne Nussbaumer, and Sandrine Fleury-Souverain, and Lucie Bouchoud, and Serge Rudaz, and Pascal Bonnabry, and Jean-Luc Veuthey
November 2005, Analytical and bioanalytical chemistry,
Copied contents to your clipboard!