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Nonlinear optical imaging of lyotropic cholesteric liquid crystals. 2010

Ariane Deniset-Besseau, and Paulo De Sa Peixoto, and Gervaise Mosser, and Marie-Claire Schanne-Klein
Laboratoire d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM U696, Palaiseau, France.

We use nonlinear optical microscopy combining Second Harmonic Generation (SHG) microscopy and Two-Photon Excited Fluorescence (2PEF) signals to characterize collagen lyotropic liquid crystals. We show that SHG signals provide highly contrasted images of the three-dimensional texture of cholesteric patterns with submicrometer lateral resolution. Moreover, simultaneous recording of the 2PEF signal enables in situ quantitative mapping of the molecular concentration and its correlation with the observed textures. We apply this technique to the characterization of biomimetic textures obtained in concentrated collagen liquid solutions. We successfully image biologically relevant organizations that are similar to the collagen organization found as a stabilized state in compact bones.

UI MeSH Term Description Entries
D007536 Isomerism The phenomenon whereby certain chemical compounds have structures that are different although the compounds possess the same elemental composition. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed) Isomerisms
D011487 Protein Conformation The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain). Conformation, Protein,Conformations, Protein,Protein Conformations
D003094 Collagen A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH). Avicon,Avitene,Collagen Felt,Collagen Fleece,Collagenfleece,Collastat,Dermodress,Microfibril Collagen Hemostat,Pangen,Zyderm,alpha-Collagen,Collagen Hemostat, Microfibril,alpha Collagen
D017711 Nonlinear Dynamics The study of systems which respond disproportionately (nonlinearly) to initial conditions or perturbing stimuli. Nonlinear systems may exhibit "chaos" which is classically characterized as sensitive dependence on initial conditions. Chaotic systems, while distinguished from more ordered periodic systems, are not random. When their behavior over time is appropriately displayed (in "phase space"), constraints are evident which are described by "strange attractors". Phase space representations of chaotic systems, or strange attractors, usually reveal fractal (FRACTALS) self-similarity across time scales. Natural, including biological, systems often display nonlinear dynamics and chaos. Chaos Theory,Models, Nonlinear,Non-linear Dynamics,Non-linear Models,Chaos Theories,Dynamics, Non-linear,Dynamics, Nonlinear,Model, Non-linear,Model, Nonlinear,Models, Non-linear,Non linear Dynamics,Non linear Models,Non-linear Dynamic,Non-linear Model,Nonlinear Dynamic,Nonlinear Model,Nonlinear Models,Theories, Chaos,Theory, Chaos
D050866 Liquid Crystals Materials in intermediate state between solid and liquid. Liquid Crystalline Materials,Mesomorphous Phase Materials,Crystal, Liquid,Crystals, Liquid,Liquid Crystal,Material, Mesomorphous Phase,Materials, Mesomorphous Phase,Mesomorphous Phase Material
D036641 Microscopy, Fluorescence, Multiphoton Fluorescence microscopy utilizing multiple low-energy photons to produce the excitation event of the fluorophore (endogenous fluorescent molecules in living tissues or FLUORESCENT DYES). Multiphoton microscopes have a simplified optical path in the emission side due to the lack of an emission pinhole, which is necessary with normal confocal microscopes. Ultimately this allows spatial isolation of the excitation event, enabling deeper imaging into optically thick tissue, while restricting photobleaching and phototoxicity to the area being imaged. Fluorescence Microscopy, Multiphoton,Multiphoton Fluorescence Microscopy,Multiphoton Excitation Microscopy,Excitation Microscopies, Multiphoton,Excitation Microscopy, Multiphoton,Microscopies, Multiphoton Excitation,Microscopy, Multiphoton Excitation,Microscopy, Multiphoton Fluorescence,Multiphoton Excitation Microscopies
D040761 Biomimetic Materials Materials fabricated by BIOMIMETICS techniques, i.e., based on natural processes found in biological systems. Bio-Inspired Devices,Bio-Inspired Materials,Bioinspired Devices,Bioinspired Materials,Biomimetic Devices,Biomimicry Devices,Biomimicry Materials,Bio Inspired Devices,Bio Inspired Materials,Bio-Inspired Device,Bio-Inspired Material,Bioinspired Device,Bioinspired Material,Biomimetic Device,Biomimetic Material,Biomimicry Device,Biomimicry Material,Device, Bio-Inspired,Device, Bioinspired,Device, Biomimetic,Device, Biomimicry,Devices, Bio-Inspired,Devices, Bioinspired,Devices, Biomimetic,Devices, Biomimicry,Material, Bio-Inspired,Material, Bioinspired,Material, Biomimetic,Material, Biomimicry,Materials, Bio-Inspired,Materials, Bioinspired,Materials, Biomimetic,Materials, Biomimicry

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