-transparent.png){kind=logo}
Mammalian farnesyltransferase, which attaches a 15 carbon isoprenoid, farnesyl, to a cysteine in p21ras proteins, contains two subunits, alpha and beta. The beta subunit is known to bind p21ras proteins. We show here that the alpha subunit is shared with another prenyltransferase that attaches 20 carbon geranylgeranyl to Ras-related proteins. Farnesyltransferase and geranylgeranyltransferase have similar molecular weights on gel filtration, but are separated by ion exchange chromatography. Both enzymes are precipitated and immunoblotted by multiple antibodies directed against the alpha subunit of farnesyltransferase. The two transferases have different specificities for the protein acceptor; farnesyltransferase prefers methionine or serine at the COOH-terminus and geranylgeranyltransferase prefers leucine. The current data indicate that both prenyltransferases are heterodimers that share a common alpha subunit with different beta subunits.
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
January 2001,
Oncogene,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
August 2006,
Biochemistry,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
February 1995,
Proceedings of the National Academy of Sciences of the United States of America,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
May 2002,
Bioorganic & medicinal chemistry letters,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
March 1994,
Biochimica et biophysica acta,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
July 2003,
Journal of medicinal chemistry,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
August 1998,
Archives of biochemistry and biophysics,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
August 1992,
The Journal of biological chemistry,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
November 1999,
The Journal of biological chemistry,
M C Seabra, and
Y Reiss, and
P J Casey, and
M S Brown, and
J L Goldstein
January 1993,
The Journal of biological chemistry,
