BIOMAT Database Logo BIOMAT Database Logo

Time-resolved luminescence-based assay for thyroglobulin. 2009

Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Institute of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 20224, Taiwan.

We have demonstrated a time-resolved luminescence (TRL) assay for the sensitive and selective detection of thyroglobulin (Tg), a thyroid cancer marker, in homogeneous solution using water-soluble alpha-D-mannose-conjugated Au nanodots (Man-Au NDs). The Man-Au NDs (1.7 nm in diameter) were prepared from the reaction between Au nanoparticles (2.9 nm in diameter) with 11-mercapto-3,6,9-trioxaundecyl-alpha-D-mannopyranoside (Man-RSH; 0-10 mM). We have found that the luminescence intensity and lifetimes of Man-Au NDs separately become stronger and longer, respectively, upon increasing the concentration of Man-RSH. The Man-Au NDs possess several interesting optical properties, including long luminescence lifetime (> 500 ns) and large Stokes' shift (> 150 nm). The TRL assay is based on the competition between Tg and Man-Au NDs for interacting with concanavalin A (Con A). The fluorescence intensities integrated over the region of 50-400 ns increase upon increasing the concentration of Tg over the range 0.1-2.5 nM. The Con A/Man-Au NDs system in a label-free manner allows the detection of Tg at concentrations as low as 90 pM in the presence of BSA at 50 microM by TRL. With its advantages of rapid and specificity, this present approach holds great potential for diagnosis of cancers.

UI MeSH Term Description Entries
D008163 Luminescent Measurements Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE. Bioluminescence Measurements,Bioluminescent Assays,Bioluminescent Measurements,Chemiluminescence Measurements,Chemiluminescent Assays,Chemiluminescent Measurements,Chemoluminescence Measurements,Luminescence Measurements,Luminescent Assays,Luminescent Techniques,Phosphorescence Measurements,Phosphorescent Assays,Phosphorescent Measurements,Assay, Bioluminescent,Assay, Chemiluminescent,Assay, Luminescent,Assay, Phosphorescent,Assays, Bioluminescent,Assays, Chemiluminescent,Assays, Luminescent,Assays, Phosphorescent,Bioluminescence Measurement,Bioluminescent Assay,Bioluminescent Measurement,Chemiluminescence Measurement,Chemiluminescent Assay,Chemiluminescent Measurement,Chemoluminescence Measurement,Luminescence Measurement,Luminescent Assay,Luminescent Measurement,Luminescent Technique,Measurement, Bioluminescence,Measurement, Bioluminescent,Measurement, Chemiluminescence,Measurement, Chemiluminescent,Measurement, Chemoluminescence,Measurement, Luminescence,Measurement, Luminescent,Measurement, Phosphorescence,Measurement, Phosphorescent,Measurements, Bioluminescence,Measurements, Bioluminescent,Measurements, Chemiluminescence,Measurements, Chemiluminescent,Measurements, Chemoluminescence,Measurements, Luminescence,Measurements, Luminescent,Measurements, Phosphorescence,Measurements, Phosphorescent,Phosphorescence Measurement,Phosphorescent Assay,Phosphorescent Measurement,Technique, Luminescent,Techniques, Luminescent
D008422 Materials Testing The testing of materials and devices, especially those used for PROSTHESES AND IMPLANTS; SUTURES; TISSUE ADHESIVES; etc., for hardness, strength, durability, safety, efficacy, and biocompatibility. Biocompatibility Testing,Biocompatible Materials Testing,Hemocompatibility Testing,Testing, Biocompatible Materials,Testing, Hemocompatible Materials,Hemocompatibility Testings,Hemocompatible Materials Testing,Materials Testing, Biocompatible,Materials Testing, Hemocompatible,Testing, Biocompatibility,Testing, Hemocompatibility,Testing, Materials,Testings, Biocompatibility
D008968 Molecular Conformation The characteristic three-dimensional shape of a molecule. Molecular Configuration,3D Molecular Structure,Configuration, Molecular,Molecular Structure, Three Dimensional,Three Dimensional Molecular Structure,3D Molecular Structures,Configurations, Molecular,Conformation, Molecular,Conformations, Molecular,Molecular Configurations,Molecular Conformations,Molecular Structure, 3D,Molecular Structures, 3D,Structure, 3D Molecular,Structures, 3D Molecular
D010316 Particle Size Relating to the size of solids. Particle Sizes,Size, Particle,Sizes, Particle
D003460 Crystallization The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Crystalline Polymorphs,Polymorphism, Crystallization,Crystal Growth,Polymorphic Crystals,Crystal, Polymorphic,Crystalline Polymorph,Crystallization Polymorphism,Crystallization Polymorphisms,Crystals, Polymorphic,Growth, Crystal,Polymorph, Crystalline,Polymorphic Crystal,Polymorphisms, Crystallization,Polymorphs, Crystalline
D006046 Gold A yellow metallic element with the atomic symbol Au, atomic number 79, and atomic weight 197. It is used in jewelry, goldplating of other metals, as currency, and in dental restoration. Many of its clinical applications, such as ANTIRHEUMATIC AGENTS, are in the form of its salts.
D001681 Biological Assay A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc. Bioassay,Assay, Biological,Assays, Biological,Biologic Assay,Biologic Assays,Assay, Biologic,Assays, Biologic,Bioassays,Biological Assays
D013499 Surface Properties Characteristics or attributes of the outer boundaries of objects, including molecules. Properties, Surface,Property, Surface,Surface Property
D013954 Thyroglobulin
D015374 Biosensing Techniques Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal. Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing

Related Publications

Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Lanthanide-based time-resolved luminescence immunoassays.
July 2011, Analytical and bioanalytical chemistry,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Single-label time-resolved luminescence assay for estrogen receptor--ligand binding.
August 2011, Analytical biochemistry,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity.
January 2015, Chemical communications (Cambridge, England),
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Homogeneous single-label biochemical Ras activation assay using time-resolved luminescence.
December 2011, Analytical chemistry,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Time-resolved circularly polarized luminescence of Eu3+ -based systems.
March 2021, Chirality,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Time-resolved fluorescence aptamer-based sandwich assay for thrombin detection.
November 2010, Talanta,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Time-resolved luminescence from annealed quartz.
January 2002, Radiation protection dosimetry,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Mito-Specific Ratiometric Terbium(III)-Complex-Based Luminescent Probe for Accurate Detection of Endogenous Peroxynitrite by Time-Resolved Luminescence Assay.
October 2019, Analytical chemistry,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Ratiometric iridium(III) complex-based phosphorescent chemodosimeter for Hg(2+) applicable in time-resolved luminescence assay and live cell imaging.
March 2015, Analytical chemistry,
Chih-Ching Huang, and Tai-Yuan Lin, and Wen-Shen Yu, and Chao-Tsen Chen, and Huan-Tsung Chang
Rapid time-resolved luminescence based screening of bacteria in urine with luminescence modulating biosensing phages.
April 2019, Analytical biochemistry,
Copied contents to your clipboard!