BIOMAT Database Logo BIOMAT Database Logo

Imaging Caenorhabditis elegans embryogenesis by third-harmonic generation microscopy. 2010

G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Institute of Electronic Structure and Laser, Foundation of Research and Technology-Hellas, P.O. Box 1385, 71110, Heraklion, Crete, Greece.

In this study, third-harmonic generation (THG) imaging measurements were performed to characterize different developmental stages of the nematode Caenorhabditis elegans (C. elegans) embryos. Femtosecond laser pulses (1028 nm) were utilized for excitation. THG image contrast modality proved as a powerful diagnostic tool, providing valuable information and offering new insights into the complex developmental process of C. elegans embryogenesis.

UI MeSH Term Description Entries
D007091 Image Processing, Computer-Assisted A technique of inputting two-dimensional or three-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer. Biomedical Image Processing,Computer-Assisted Image Processing,Digital Image Processing,Image Analysis, Computer-Assisted,Image Reconstruction,Medical Image Processing,Analysis, Computer-Assisted Image,Computer-Assisted Image Analysis,Computer Assisted Image Analysis,Computer Assisted Image Processing,Computer-Assisted Image Analyses,Image Analyses, Computer-Assisted,Image Analysis, Computer Assisted,Image Processing, Biomedical,Image Processing, Computer Assisted,Image Processing, Digital,Image Processing, Medical,Image Processings, Medical,Image Reconstructions,Medical Image Processings,Processing, Biomedical Image,Processing, Digital Image,Processing, Medical Image,Processings, Digital Image,Processings, Medical Image,Reconstruction, Image,Reconstructions, Image
D007834 Lasers An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum. Masers,Continuous Wave Lasers,Pulsed Lasers,Q-Switched Lasers,Continuous Wave Laser,Laser,Laser, Continuous Wave,Laser, Pulsed,Laser, Q-Switched,Lasers, Continuous Wave,Lasers, Pulsed,Lasers, Q-Switched,Maser,Pulsed Laser,Q Switched Lasers,Q-Switched Laser
D008853 Microscopy The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe. Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D004625 Embryo, Nonmammalian The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO. Embryonic Structures, Nonmammalian,Embryo, Non-Mammalian,Embryonic Structures, Non-Mammalian,Nonmammalian Embryo,Nonmammalian Embryo Structures,Nonmammalian Embryonic Structures,Embryo Structure, Nonmammalian,Embryo Structures, Nonmammalian,Embryo, Non Mammalian,Embryonic Structure, Non-Mammalian,Embryonic Structure, Nonmammalian,Embryonic Structures, Non Mammalian,Embryos, Non-Mammalian,Embryos, Nonmammalian,Non-Mammalian Embryo,Non-Mammalian Embryonic Structure,Non-Mammalian Embryonic Structures,Non-Mammalian Embryos,Nonmammalian Embryo Structure,Nonmammalian Embryonic Structure,Nonmammalian Embryos,Structure, Non-Mammalian Embryonic,Structure, Nonmammalian Embryo,Structure, Nonmammalian Embryonic,Structures, Non-Mammalian Embryonic,Structures, Nonmammalian Embryo,Structures, Nonmammalian Embryonic
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D017173 Caenorhabditis elegans A species of nematode that is widely used in biological, biochemical, and genetic studies. Caenorhabditis elegan,elegan, Caenorhabditis
D047108 Embryonic Development Morphological and physiological development of EMBRYOS. Embryo Development,Embryogenesis,Postimplantation Embryo Development,Preimplantation Embryo Development,Embryonic Programming,Post-implantation Embryo Development,Postnidation Embryo Development,Postnidation Embryo Development, Animal,Pre-implantation Embryo Development,Prenidation Embryo Development, Animal,Development, Embryo,Development, Embryonic,Development, Postnidation Embryo,Embryo Development, Post-implantation,Embryo Development, Postimplantation,Embryo Development, Postnidation,Embryo Development, Pre-implantation,Embryo Development, Preimplantation,Embryonic Developments,Embryonic Programmings,Post implantation Embryo Development,Pre implantation Embryo Development

Related Publications

G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
In vivo imaging of neurodegeneration in Caenorhabditis elegans by third harmonic generation microscopy.
November 2008, Journal of microscopy,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Third-harmonic generation for the study of Caenorhabditis elegans embryogenesis.
January 2010, Journal of biomedical optics,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Depth-resolved structural imaging by third-harmonic generation microscopy.
July 2004, Journal of structural biology,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Third harmonic generation microscopy.
October 1998, Optics express,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Imaging granularity of leukocytes with third harmonic generation microscopy.
September 2012, Biomedical optics express,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Imaging of Caenorhabditis elegans neurons by second-harmonic generation and two-photon excitation fluorescence.
January 2005, Journal of biomedical optics,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Label-free imaging of Schwann cell myelination by third harmonic generation microscopy.
December 2014, Proceedings of the National Academy of Sciences of the United States of America,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Tripling the maximum imaging depth with third-harmonic generation microscopy.
September 2015, Journal of biomedical optics,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Imaging Cytometry of Human Leukocytes with Third Harmonic Generation Microscopy.
November 2016, Scientific reports,
G J Tserevelakis, and G Filippidis, and A J Krmpot, and M Vlachos, and C Fotakis, and N Tavernarakis
Time-lapse microscopy of early embryogenesis in Caenorhabditis elegans.
August 2011, Journal of visualized experiments : JoVE,
Copied contents to your clipboard!