BIOMAT Database Logo BIOMAT Database Logo

Heterologous expression of the oxytetracycline biosynthetic pathway in Myxococcus xanthus. 2010

D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Department of Chemistry, Syracuse University, Syracuse, New York 132441, USA.

New natural products for drug discovery may be accessed by heterologous expression of bacterial biosynthetic pathways in metagenomic DNA libraries. However, a "universal" host is needed for this experiment. Herein, we show that Myxococcus xanthus is a potential "universal" host for heterologous expression of polyketide biosynthetic gene clusters.

UI MeSH Term Description Entries
D010118 Oxytetracycline A TETRACYCLINE analog isolated from the actinomycete STREPTOMYCES RIMOSUS and used in a wide variety of clinical conditions. Hydroxytetracycline,Bisolvomycin,Geomycin,Oxyterracin,Oxyterracine,Oxytetracid,Oxytetracycline Anhydrous,Oxytetracycline Calcium,Oxytetracycline Dihydrate,Oxytetracycline Hydrochloride,Oxytetracycline Monohydrochloride,Oxytetracycline Sulfate (2:1),Oxytetracycline, (4a beta,5 beta,5a beta,12a beta)-Isomer,Oxytetracycline, (5 beta)-Isomer,Oxytetracycline, Anhydrous,Oxytetracycline, Calcium (1:1) Salt,Oxytetracycline, Disodium Salt, Dihydrate,Oxytetracycline, Sodium Salt,Terramycin
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D005810 Multigene Family A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed) Gene Clusters,Genes, Reiterated,Cluster, Gene,Clusters, Gene,Families, Multigene,Family, Multigene,Gene Cluster,Gene, Reiterated,Multigene Families,Reiterated Gene,Reiterated Genes
D001426 Bacterial Proteins Proteins found in any species of bacterium. Bacterial Gene Products,Bacterial Gene Proteins,Gene Products, Bacterial,Bacterial Gene Product,Bacterial Gene Protein,Bacterial Protein,Gene Product, Bacterial,Gene Protein, Bacterial,Gene Proteins, Bacterial,Protein, Bacterial,Proteins, Bacterial
D016941 Myxococcus xanthus A species of gliding bacteria found on soil as well as in surface fresh water and coastal seawater.
D053898 Biosynthetic Pathways Sets of enzymatic reactions occurring in organisms and that form biochemicals by making new covalent bonds. Biosynthetic Pathway,Pathway, Biosynthetic,Pathways, Biosynthetic
D023061 Gene Order The sequential location of genes on a chromosome. Gene Arrangement,Gene Position,Arrangement, Gene,Arrangements, Gene,Gene Arrangements,Gene Positions,Order, Gene,Position, Gene,Positions, Gene

Related Publications

D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Heterologous expression of epothilone biosynthetic genes in Myxococcus xanthus.
September 2002, Antimicrobial agents and chemotherapy,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Reconstitution of the myxothiazol biosynthetic gene cluster by Red/ET recombination and heterologous expression in Myxococcus xanthus.
December 2006, Applied and environmental microbiology,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Optimizing the heterologous production of epothilone D in Myxococcus xanthus.
May 2002, Biotechnology and bioengineering,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Heterologous production of myxobacterial α-pyrone antibiotics in Myxococcus xanthus.
November 2017, Metabolic engineering,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Characterization of the Exopolysaccharide Biosynthesis Pathway in Myxococcus xanthus.
September 2020, Journal of bacteriology,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
csgA expression entrains Myxococcus xanthus development.
March 1992, Genes & development,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Myxococcus xanthus.
June 2021, Trends in microbiology,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Identification and characterization of the althiomycin biosynthetic gene cluster in Myxococcus xanthus DK897.
June 2011, Chembiochem : a European journal of chemical biology,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Light induction of gene expression in Myxococcus xanthus.
April 1987, Proceedings of the National Academy of Sciences of the United States of America,
D Cole Stevens, and Michael R Henry, and Kimberly A Murphy, and Christopher N Boddy
Characterization of four type IV pilin homologues in Stigmatella aurantiaca DSM17044 by heterologous expression in Myxococcus xanthus.
January 2013, PloS one,
Copied contents to your clipboard!