Novel microRNAs in silkworm (Bombyx mori). 2010

Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, 100029, China. caiyimei_001@163.com

We acquired more than 4 million useful sequences using a high-throughput method from a library for miRNA identification, which is constructed from a mixture of 14 RNA samples from different developmental stages. We mapped 247,410 reads to known silkworm miRNAs in miRBase (13.0), 701,913 reads to other RNA molecules based on sequence homology, and 3,219,395 reads to the silkworm genome. Our analysis identified 54 silkworm known miRNAs. A striking strand bias between miRNAs and their corresponding miRNA*s was found, and was speculated to reflect that transcripts from the passenger strand of pre-miRNAs may have important biological roles. Using an elaborate screening protocol, we predicted 287 candidate novel miRNAs (represent 116,494 short reads), and 59 of them have both miRNA and miRNA* sequences. Most of the previously identified silkworm miRNAs are cross-species conserved with a high abundance, while those predicted candidates tend to be species-specific miRNAs. Our discovery of SNPs among miRNAs implied within-species functional diversity. Target prediction uncovers that considerable silkworm miRNAs may aim at modulating more than one hormone signaling pathway components and/or hormone biosynthesis-related proteins implying their important roles in silkworm development.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009690 Nucleic Acid Conformation The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape. DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012831 Bombyx A genus of silkworm MOTHS in the family Bombycidae of the order LEPIDOPTERA. The family contains a single species, Bombyx mori from the Greek for silkworm + mulberry tree (on which it feeds). A native of Asia, it is sometimes reared in this country. It has long been raised for its SILK and after centuries of domestication it probably does not exist in nature. It is used extensively in experimental GENETICS. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p519) Bombyx mori,Silkmoths,Silkworms,Silkmoth,Silkworm,Bombyx morus,Bombyxs,mori, Bombyx
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D015203 Reproducibility of Results The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results. Reliability and Validity,Reliability of Result,Reproducibility Of Result,Reproducibility of Finding,Validity of Result,Validity of Results,Face Validity,Reliability (Epidemiology),Reliability of Results,Reproducibility of Findings,Test-Retest Reliability,Validity (Epidemiology),Finding Reproducibilities,Finding Reproducibility,Of Result, Reproducibility,Of Results, Reproducibility,Reliabilities, Test-Retest,Reliability, Test-Retest,Result Reliabilities,Result Reliability,Result Validities,Result Validity,Result, Reproducibility Of,Results, Reproducibility Of,Test Retest Reliability,Validity and Reliability,Validity, Face
D015723 Gene Library A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences. DNA Library,cDNA Library,DNA Libraries,Gene Libraries,Libraries, DNA,Libraries, Gene,Libraries, cDNA,Library, DNA,Library, Gene,Library, cDNA,cDNA Libraries
D016000 Cluster Analysis A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both. Clustering,Analyses, Cluster,Analysis, Cluster,Cluster Analyses,Clusterings

Related Publications

Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
October 2010, Molecular biology reports,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
January 2019, Biological procedures online,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
July 2020, Archives of insect biochemistry and physiology,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
February 2002, European journal of biochemistry,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
May 1992, Experientia,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
January 2018, The Journal of toxicological sciences,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
January 1953, Advances in genetics,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
October 2023, Insect molecular biology,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
January 2013, PloS one,
Yimei Cai, and Xiaomin Yu, and Qing Zhou, and Caixia Yu, and Haiyan Hu, and Jiucheng Liu, and Hongbin Lin, and Jin Yang, and Bing Zhang, and Peng Cui, and Songnian Hu, and Jun Yu
May 2011, BMC genomics,
Copied contents to your clipboard!