-transparent.png){kind=logo}
The 35S rRNA gene of the yeast Saccharomyces cerevisiae was fused to the GAL7 promoter. This hybrid gene, when present on a multicopy plasmid and induced by galactose, suppressed the growth defects of a temperature-sensitive RNA polymerase I (pol I) mutant and those of a mutant in which the gene for the second largest subunit of pol I was deleted. Analysis of pulse-labeled RNA directly demonstrated that rRNA synthesis in this deletion mutant is from the GAL7 promoter. These experiments show that the sole essential function of pol I is the transcription of the rRNA genes, that pol I is not absolutely required for the synthesis of rRNA and ribosomes or cell growth if 35S rRNA synthesis is achieved by some other means, and that the tandemly repeated structure of the chromosomal rRNA genes is also not absolutely required for the synthesis of rRNA and ribosomes.
Y Nogi, and
R Yano, and
M Nomura
April 1993,
Molecular and cellular biology,
Y Nogi, and
R Yano, and
M Nomura
November 1997,
Nucleic acids research,
Y Nogi, and
R Yano, and
M Nomura
June 1979,
Molecular & general genetics : MGG,
Y Nogi, and
R Yano, and
M Nomura
June 1987,
Molecular and cellular biology,
Y Nogi, and
R Yano, and
M Nomura
August 1991,
Proceedings of the National Academy of Sciences of the United States of America,
Y Nogi, and
R Yano, and
M Nomura
January 2001,
Structure (London, England : 1993),
Y Nogi, and
R Yano, and
M Nomura
June 1986,
Molecular and cellular biology,
Y Nogi, and
R Yano, and
M Nomura
January 1989,
Yeast (Chichester, England),
Y Nogi, and
R Yano, and
M Nomura
August 1989,
Molecular and cellular biology,
Y Nogi, and
R Yano, and
M Nomura
January 1979,
Molecular & general genetics : MGG,
