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The effects of PAF (1-2 X 10(-7) M) on intracellular slow Ca-mediated action potentials (Ca-AP) and isometric contractions of guinea-pig auricle muscles and ionic currents of frog atrial trabeculae were investigated. PAF decreased the amplitude, duration and upstroke velocity of the slow Ca-AP mediated via the L-type Ca channels in guinea-pig auricle muscles without changes in the resting potential. The PAF receptor antagonist U-66985 (1-5 X 10(-6) M), histamine (10(-4) M) and 4-aminopyridine (3 mM) inhibited the depressant effects of PAF on the slow AP and contractions. PAF reduced the peak of the slow inward Ca current (ICa) via the L-type Ca channels of frog atrial trabeculae. Simultaneously, the delayed outward K current (IK) was increased by PAF. Pretreatment with the PAF receptor antagonist BN 52021 (4 X 10(-6) M) protected the effects of PAF on ICa and IK. Our data demonstrate both by direct and indirect methods of ICa registration an inhibiting effect of PAF on the ICa via the L-type Ca channels and make probable a similarity in PAF signalling in cardiac cells of cold- and warm-blooded animals. The coupling of changes of the ICa with the augmentation of IK accentuate the important role of myocardial L-type Ca and K channels in mediating the negative inotropic action of PAF. The ability of U-66985 and BN 52021 to inhibit the electrophysiological effects of PAF hint at a membrane signalling coupled by specific binding sites for PAF in the myocardium.
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
June 1997,
Magnesium research,
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
September 2006,
The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation,
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
January 1975,
Kardiologia polska,
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
February 1959,
Archives des maladies du coeur et des vaisseaux,
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
February 1997,
European journal of pharmacology,
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
January 1989,
Journal of lipid mediators,
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
December 1985,
Clinical science (London, England : 1979),
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
September 2013,
European journal of pain (London, England),
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
January 1987,
International journal of tissue reactions,
M Gollasch, and
V B Ignatieva, and
E M Kobrinsky, and
E G Vornovitsky
July 2003,
British journal of pharmacology,
