Detection of Chlamydia trachomatis by the polymerase chain reaction in swabs and urine from men with non-gonococcal urethritis. 1991

H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex.

A polymerase chain reaction (PCR) was developed for Chlamydia trachomatis in which a 380 base pair DNA fragment was amplified. Amplification occurred with the DNA from the 15 serovars but not with that from other Chlamydia spp or with DNA from a variety of other organisms. Chlamydial DNA (10(-16) g) could be detected and the PCR seemed to be able to detect single organisms. Urethral swabs were obtained from 37 men with acute non-gonococcal urethritis (NGU), 18 (49%) of whom were positive for C trachomatis by MicroTrak. As a result of clinical re-examinations 65 urethral swabs were available for analysis by the PCR. In comparison with MicroTrak, PCR had a sensitivity of 95%, a specificity of 94%, a positive predictive value of 86% and a negative predictive value of 98%. The PCR was apparently less sensitive (82%) in tests on urine samples. Overall, however, values of sensitivity and specificity of the PCR compared favourably with those of MicroTrak. The PCR for C trachomatis is likely to be a valuable technique for research, but problems of DNA contamination suggest that it should not be recommended for routine diagnosis.

UI MeSH Term Description Entries
D008297 Male Males
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D011237 Predictive Value of Tests In screening and diagnostic tests, the probability that a person with a positive test is a true positive (i.e., has the disease), is referred to as the predictive value of a positive test; whereas, the predictive value of a negative test is the probability that the person with a negative test does not have the disease. Predictive value is related to the sensitivity and specificity of the test. Negative Predictive Value,Positive Predictive Value,Predictive Value Of Test,Predictive Values Of Tests,Negative Predictive Values,Positive Predictive Values,Predictive Value, Negative,Predictive Value, Positive
D002690 Chlamydia Infections Infections with bacteria of the genus CHLAMYDIA. Infections, Chlamydia,Chlamydia Infection,Infection, Chlamydia
D002692 Chlamydia trachomatis Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.
D004587 Electrophoresis, Agar Gel Electrophoresis in which agar or agarose gel is used as the diffusion medium. Electrophoresis, Agarose Gel,Agar Gel Electrophoresis,Agarose Gel Electrophoresis,Gel Electrophoresis, Agar,Gel Electrophoresis, Agarose
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D014521 Urethra A tube that transports URINE from the URINARY BLADDER to the outside of the body in both the sexes. It also has a reproductive function in the male by providing a passage for SPERM. External Urethral Sphincter,External Urinary Sphincter,Internal Urethral Sphincter,Internal Urinary Sphincter,Internal Vesical Sphincter,Urethral Sphincters,External Urethral Sphincters,External Urinary Sphincters,Internal Urethral Sphincters,Internal Urinary Sphincters,Internal Vesical Sphincters,Sphincter, External Urethral,Sphincter, External Urinary,Sphincter, Internal Urethral,Sphincter, Internal Urinary,Sphincter, Internal Vesical,Sphincter, Urethral,Urethral Sphincter,Urethral Sphincter, External,Urethras,Urinary Sphincter, External,Urinary Sphincter, Internal,Vesical Sphincter, Internal

Related Publications

H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
July 1991, Journal of clinical pathology,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
August 2004, Sexually transmitted infections,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
July 1992, Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
November 1993, Journal of clinical microbiology,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
August 2002, Sexually transmitted infections,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
June 1993, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
December 1993, European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
December 1991, Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
June 1997, The British journal of ophthalmology,
H M Palmer, and C B Gilroy, and B J Thomas, and P E Hay, and C Gilchrist, and D Taylor-Robinson
March 1980, Ugeskrift for laeger,
Copied contents to your clipboard!