-transparent.png){kind=logo}
Previous studies have shown that the application of Ad/AFPtBid significantly and specifically killed hepatocellular carcinoma (HCC) cells in culture and subcutaneously implanted in mice. This study was to test the therapeutic efficacy of Ad/AFPtBid in an orthotopic hepatic tumor model. Four weeks after implantation of tumor cells into the liver, nude mice were treated with Ad/AFPtBid alone or in combination with 5-fluorouracil (5-FU). Serum alpha-fetoprotein (AFP) was measured as a marker for tumor progression. The results showed that Ad/AFPtBid significantly inhibited Hep3B tumor growth. Ad/AFPtBid and 5-FU in combination was more effective than either agent alone. Tumor tissues of Ad/AFPtBid alone or combination treatment groups showed a decrease in cells positive for proliferation cell nuclear antigen, but an increase in apoptosis. Ad/AFPtBid did not suppress the hepatic tumor formed by non-AFP-producing hepatoma SK-HEP-1 cells or colorectal adenocarcinoma DLD-1 cells. The survival rate was higher in mice treated with Ad/AFPtBid plus 5-FU than those treated with either agent alone. No acute toxic effect was observed in mice receiving Ad/AFPtBid. Collectively, Ad/AFPtBid can specifically target and effectively suppress the AFP-producing orthotopic liver tumor in mice without obvious toxicity, indicating that it is a promising tool in combination with chemotherapeutic agents for treatment of AFP-producing HCC.
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
March 2015,
BMC cancer,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
June 2014,
Experimental cell research,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
November 1978,
Journal of the National Cancer Institute,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
April 1989,
British journal of cancer,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
December 2016,
Biochemistry. Biokhimiia,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
November 1961,
Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.),
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
February 1976,
Cancer,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
January 1987,
Virchows Archiv. A, Pathological anatomy and histopathology,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
February 1977,
Biulleten' eksperimental'noi biologii i meditsiny,
S-H Ma, and
G G Chen, and
J Yip, and
P B S Lai
January 1979,
Revista medica de Chile,
